145457

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3657930..3658432 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GRF62_RS17365 Protein ID WP_000767829.1
Coordinates 3658178..3658432 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GRF62_RS17360 Protein ID WP_001259255.1
Coordinates 3657930..3658181 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS17335 3653108..3654175 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GRF62_RS17340 3654175..3655245 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
GRF62_RS17345 3655242..3656546 - 1305 WP_000009594.1 histidinol dehydrogenase -
GRF62_RS17350 3656552..3657451 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GRF62_RS17355 3657597..3657647 - 51 WP_001364200.1 his operon leader peptide -
GRF62_RS17360 3657930..3658181 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GRF62_RS17365 3658178..3658432 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GRF62_RS17370 3658515..3659339 + 825 WP_000754737.1 SDR family oxidoreductase -
GRF62_RS17375 3659385..3660314 + 930 WP_000803351.1 LysR family transcriptional regulator -
GRF62_RS17380 3660529..3660591 + 63 WP_010723108.1 membrane protein YoeI -
GRF62_RS17385 3660581..3661685 + 1105 Protein_3374 APC family permease -
GRF62_RS17390 3661864..3662922 + 1059 WP_000492339.1 transport protein YeeE -
GRF62_RS17395 3662936..3663163 + 228 WP_046613325.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T145457 WP_000767829.1 NZ_CP047127:3658178-3658432 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T145457 NZ_CP062388:20152-20328 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT145457 WP_001259255.1 NZ_CP047127:3657930-3658181 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT145457 NZ_CP062388:c20490-20316 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References