145451

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2519164..2519891 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag GRF62_RS12060 Protein ID WP_000550189.1
Coordinates 2519164..2519478 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GRF62_RS12065 Protein ID WP_000560266.1
Coordinates 2519475..2519891 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS12040 2515322..2516308 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
GRF62_RS12045 2516387..2517079 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
GRF62_RS12050 2517156..2517659 - 504 WP_001333820.1 M48 family metallopeptidase -
GRF62_RS12055 2517744..2518880 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
GRF62_RS12060 2519164..2519478 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
GRF62_RS12065 2519475..2519891 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GRF62_RS12070 2519936..2521954 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
GRF62_RS12075 2522380..2524731 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T145451 WP_000550189.1 NZ_CP047127:2519164-2519478 [Escherichia coli K-12]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T145451 NZ_CP062386:2311246-2311353 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT145451 WP_000560266.1 NZ_CP047127:2519475-2519891 [Escherichia coli K-12]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT145451 NZ_CP062386:c2311430-2311370 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References