Detailed information of TA system TA145443

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 1172439..1172851
• Replicon: chromosome
• Accession: NZ_CP047127
• Organism: Escherichia coli K-12

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: GRF62_RS05655
• Protein ID: WP_000132601.1
• Coordinates: 1172510..1172851 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	1172439..1172515 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRF62_RS05645	1169302..1170891	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
GRF62_RS05650	1170888..1172282	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	1172439..1172515	-	77	NuclAT_14	-	Antitoxin
-	1172439..1172515	-	77	NuclAT_14	-	Antitoxin
-	1172439..1172515	-	77	NuclAT_14	-	Antitoxin
-	1172439..1172515	-	77	NuclAT_14	-	Antitoxin
-	1172439..1172515	-	77	NuclAT_15	-	Antitoxin
-	1172439..1172515	-	77	NuclAT_15	-	Antitoxin
-	1172439..1172515	-	77	NuclAT_15	-	Antitoxin
-	1172439..1172515	-	77	NuclAT_15	-	Antitoxin
GRF62_RS05655	1172510..1172851	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
GRF62_RS05660	1173013..1174392	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
GRF62_RS05665	1174392..1175438	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T145443 WP_000132601.1 NZ_CP047127:1172510-1172851 [Escherichia coli K-12]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T145443 NZ_CP062384:2287881-2287988 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT145443 NZ_CP047127:c1172515-1172439 [Escherichia coli K-12]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin