145442

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 864221..864762 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag GRF62_RS04235 Protein ID WP_000615983.1
Coordinates 864484..864762 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag GRF62_RS04230 Protein ID WP_000729703.1
Coordinates 864221..864481 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS04210 859896..860681 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GRF62_RS04215 860653..862365 + 1713 Protein_823 flagellar biosynthesis protein FlhA -
GRF62_RS04220 862589..863086 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
GRF62_RS04225 863262..864011 - 750 WP_000056849.1 C40 family peptidase -
GRF62_RS04230 864221..864481 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GRF62_RS04235 864484..864762 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GRF62_RS04240 864918..865658 + 741 WP_001225679.1 murein L,D-transpeptidase -
GRF62_RS04245 865629..866396 - 768 WP_000333380.1 class II glutamine amidotransferase -
GRF62_RS04250 866602..867180 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T145442 WP_000615983.1 NZ_CP047127:864484-864762 [Escherichia coli K-12]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T145442 NZ_CP062384:c1168615-1168298 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT145442 WP_000729703.1 NZ_CP047127:864221-864481 [Escherichia coli K-12]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT145442 NZ_CP062384:c1168827-1168618 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATAGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References