145441

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 858024..858718 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag GRF62_RS04195 Protein ID WP_001263489.1
Coordinates 858024..858422 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag GRF62_RS04200 Protein ID WP_000554758.1
Coordinates 858425..858718 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 853612..853692 - 81 NuclAT_12 - -
- 853612..853692 - 81 NuclAT_12 - -
- 853612..853692 - 81 NuclAT_12 - -
- 853612..853692 - 81 NuclAT_12 - -
GRF62_RS04170 854288..854746 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
GRF62_RS04175 855007..856464 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
GRF62_RS04180 856521..857042 - 522 Protein_816 peptide chain release factor H -
GRF62_RS04185 857038..857244 - 207 Protein_817 RtcB family protein -
GRF62_RS04190 857562..858014 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
GRF62_RS04195 858024..858422 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GRF62_RS04200 858425..858718 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GRF62_RS04205 858770..859825 - 1056 WP_001226164.1 DNA polymerase IV -
GRF62_RS04210 859896..860681 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
GRF62_RS04215 860653..862365 + 1713 Protein_823 flagellar biosynthesis protein FlhA -
GRF62_RS04220 862589..863086 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T145441 WP_001263489.1 NZ_CP047127:c858422-858024 [Escherichia coli K-12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T145441 NZ_CP062384:1140516-1140704 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT145441 WP_000554758.1 NZ_CP047127:c858718-858425 [Escherichia coli K-12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT145441 NZ_CP062384:1140227-1140487 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References