Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 239026..239619 Replicon chromosome
Accession NZ_CP047073
Organism Pseudomonas syringae pv. tomato strain delta X

Toxin (Protein)


Gene name graT Uniprot ID Q88B20
Locus tag GRB21_RS01145 Protein ID WP_011103035.1
Coordinates 239026..239304 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88B19
Locus tag GRB21_RS01150 Protein ID WP_005739893.1
Coordinates 239314..239619 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRB21_RS01125 234287..235525 - 1239 WP_011103031.1 diaminopimelate decarboxylase -
GRB21_RS01130 235527..236402 - 876 WP_011103032.1 DMT family transporter -
GRB21_RS01135 236402..237685 - 1284 WP_011103033.1 lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein -
GRB21_RS01140 237840..238775 + 936 WP_011103034.1 LysR family transcriptional regulator -
GRB21_RS01145 239026..239304 + 279 WP_011103035.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GRB21_RS01150 239314..239619 + 306 WP_005739893.1 HigA family addiction module antidote protein Antitoxin
GRB21_RS01155 239653..239934 - 282 WP_005768515.1 accessory factor UbiK family protein -
GRB21_RS01165 240354..240692 + 339 WP_002555808.1 P-II family nitrogen regulator -
GRB21_RS01170 240728..242065 + 1338 WP_005739890.1 ammonium transporter -
GRB21_RS01175 242307..242732 + 426 WP_005768513.1 secondary thiamine-phosphate synthase enzyme YjbQ -
GRB21_RS01180 242831..243160 + 330 WP_005768511.1 hypothetical protein -
GRB21_RS01185 243235..243927 - 693 WP_005768510.1 HAD family hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10453.11 Da        Isoelectric Point: 9.1490

>T145410 WP_011103035.1 NZ_CP047073:239026-239304 [Pseudomonas syringae pv. tomato]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEALDGSRSGQYSVRINAPFRICFVWSI
NGPEDVEIVDYH

Download         Length: 279 bp

>T145410 NZ_CP062378:c531529-531341 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11158.01 Da        Isoelectric Point: 7.7509

>AT145410 WP_005739893.1 NZ_CP047073:239314-239619 [Pseudomonas syringae pv. tomato]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Download         Length: 306 bp

>AT145410 NZ_CP062378:c531818-531558 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88B20

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LYY6

References