Detailed information of TA system TA145386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4480561..4481163
• Replicon: chromosome
• Accession: NZ_CP047057
• Organism: Escherichia coli NC101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: GQ584_RS21550
• Protein ID: WP_000897302.1
• Coordinates: 4480561..4480872 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GQ584_RS21555
• Protein ID: WP_000356397.1
• Coordinates: 4480873..4481163 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ584_RS21525 (4476475)	4476475..4477074	+	600	WP_001314324.1	glucose-1-phosphatase	-
GQ584_RS21530 (4477068)	4477068..4477940	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GQ584_RS21535 (4477937)	4477937..4478374	+	438	WP_000560990.1	D-aminoacyl-tRNA deacylase	-
GQ584_RS21540 (4478419)	4478419..4479360	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GQ584_RS21545 (4479424)	4479424..4480332	-	909	WP_001331553.1	alpha/beta hydrolase	-
GQ584_RS21550 (4480561)	4480561..4480872	+	312	WP_000897302.1	hypothetical protein	Toxin
GQ584_RS21555 (4480873)	4480873..4481163	+	291	WP_000356397.1	NadS family protein	Antitoxin
GQ584_RS21560 (4481522)	4481522..4481800	+	279	WP_001296612.1	hypothetical protein	-
GQ584_RS21565 (4482196)	4482196..4482414	+	219	WP_001314326.1	CopG family transcriptional regulator	-
GQ584_RS21570 (4482630)	4482630..4483559	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
GQ584_RS21575 (4483556)	4483556..4484191	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GQ584_RS21580 (4484188)	4484188..4485090	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T145386 WP_000897302.1 NZ_CP047057:4480561-4480872 [Escherichia coli NC101]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T145386 NZ_CP062370:2262292-2262558 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT145386 WP_000356397.1 NZ_CP047057:4480873-4481163 [Escherichia coli NC101]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT145386 NZ_CP062370:2262035-2262292 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: S1P416