Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3667108..3667907 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag GQ584_RS17590 Protein ID WP_000347251.1
Coordinates 3667443..3667907 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag GQ584_RS17585 Protein ID WP_001296435.1
Coordinates 3667108..3667443 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS17570 (3662893) 3662893..3663663 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GQ584_RS17575 (3663679) 3663679..3665013 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
GQ584_RS17580 (3665388) 3665388..3666959 + 1572 WP_001273939.1 galactarate dehydratase -
GQ584_RS17585 (3667108) 3667108..3667443 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GQ584_RS17590 (3667443) 3667443..3667907 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GQ584_RS17595 (3667962) 3667962..3668771 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GQ584_RS17600 (3669020) 3669020..3670300 + 1281 WP_000681930.1 tagatose-bisphosphate aldolase subunit KbaZ -
GQ584_RS17605 (3670323) 3670323..3670796 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
GQ584_RS17610 (3670807) 3670807..3671586 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
GQ584_RS17615 (3671576) 3671576..3672454 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
GQ584_RS17620 (3672472) 3672472..3672906 + 435 WP_000948834.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T145385 WP_000347251.1 NZ_CP047057:3667443-3667907 [Escherichia coli NC101]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T145385 NZ_CP062370:1153520-1153708 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT145385 WP_001296435.1 NZ_CP047057:3667108-3667443 [Escherichia coli NC101]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT145385 NZ_CP062370:1153231-1153491 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References