Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1735857..1736410 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag GQ584_RS08540 Protein ID WP_000605680.1
Coordinates 1736141..1736410 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag GQ584_RS08535 Protein ID WP_000781370.1
Coordinates 1735857..1736141 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS08520 (1731038) 1731038..1734085 + 3048 WP_077634977.1 formate dehydrogenase-N subunit alpha -
GQ584_RS08525 (1734098) 1734098..1734982 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
GQ584_RS08530 (1734975) 1734975..1735628 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
GQ584_RS08535 (1735857) 1735857..1736141 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
GQ584_RS08540 (1736141) 1736141..1736410 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GQ584_RS08545 (1736596) 1736596..1737606 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
GQ584_RS08550 (1737740) 1737740..1739437 - 1698 WP_000433464.1 malate dehydrogenase -
GQ584_RS08555 (1739594) 1739594..1739731 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
GQ584_RS08560 (1739833) 1739833..1740048 - 216 WP_000495766.1 biofilm-dependent modulation protein -
GQ584_RS08565 (1740393) 1740393..1740824 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T145371 WP_000605680.1 NZ_CP047057:c1736410-1736141 [Escherichia coli NC101]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T145371 NZ_CP062366:2844716-2845078 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT145371 WP_000781370.1 NZ_CP047057:c1736141-1735857 [Escherichia coli NC101]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT145371 NZ_CP062366:2844549-2844719 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References