Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 440809..441503 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag GQ584_RS02095 Protein ID WP_001263501.1
Coordinates 441105..441503 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag GQ584_RS02090 Protein ID WP_000554755.1
Coordinates 440809..441102 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS02070 (436523) 436523..437095 + 573 WP_000086540.1 recombinase family protein -
GQ584_RS02075 (437099) 437099..437824 - 726 WP_226226728.1 hypothetical protein -
GQ584_RS02080 (438099) 438099..439643 - 1545 WP_000758648.1 recombinase family protein -
GQ584_RS02085 (439726) 439726..440757 + 1032 WP_032150137.1 DNA polymerase IV -
GQ584_RS02090 (440809) 440809..441102 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GQ584_RS02095 (441105) 441105..441503 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GQ584_RS02100 (441513) 441513..441965 + 453 WP_001059897.1 GNAT family N-acetyltransferase -
GQ584_RS02105 (442155) 442155..443294 + 1140 WP_000521578.1 RNA ligase RtcB family protein -
GQ584_RS02110 (443291) 443291..443905 + 615 WP_000602123.1 peptide chain release factor H -
GQ584_RS02115 (443962) 443962..445419 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
GQ584_RS02120 (445680) 445680..446138 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T145368 WP_001263501.1 NZ_CP047057:441105-441503 [Escherichia coli NC101]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T145368 NZ_CP062366:2501434-2501700 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT145368 WP_000554755.1 NZ_CP047057:440809-441102 [Escherichia coli NC101]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT145368 NZ_CP062366:2501183-2501434 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGTTATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References