Detailed information of TA system TA145328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 76772..77483
• Replicon: plasmid ptetA
• Accession: NZ_CP047013
• Organism: Escherichia coli strain 19-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: GQF95_RS24825
• Protein ID: WP_000162415.1
• Coordinates: 76772..77074 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GQF95_RS24830
• Protein ID: WP_032220986.1
• Coordinates: 77145..77483 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF95_RS24795 (GQF95_24765)	71791..72441	-	651	WP_212483573.1	hypothetical protein	-
GQF95_RS24800 (GQF95_24770)	72455..73360	-	906	WP_212483557.1	recombination-associated protein RdgC	-
GQF95_RS24805 (GQF95_24775)	73420..74073	-	654	WP_212483559.1	baseplate protein	-
GQF95_RS24810 (GQF95_24780)	74070..75050	-	981	WP_000046500.1	hypothetical protein	-
GQF95_RS24815 (GQF95_24785)	75054..75821	-	768	WP_000203292.1	hypothetical protein	-
GQF95_RS24820 (GQF95_24790)	75818..76609	-	792	WP_212483561.1	phage tail protein	-
GQF95_RS24825 (GQF95_24795)	76772..77074	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GQF95_RS24830 (GQF95_24800)	77145..77483	+	339	WP_032220986.1	HigA family addiction module antitoxin	Antitoxin
GQF95_RS24835 (GQF95_24805)	77540..77725	+	186	Protein_81	hypothetical protein	-
GQF95_RS24840 (GQF95_24810)	77762..78490	+	729	WP_212483563.1	hypothetical protein	-
GQF95_RS24845 (GQF95_24815)	78524..79717	-	1194	WP_212483565.1	phage tail protein	-
GQF95_RS24850 (GQF95_24820)	79710..80039	-	330	WP_000542383.1	hypothetical protein	-
GQF95_RS24855 (GQF95_24825)	80368..81021	-	654	WP_077881123.1	maturation control protein	-
GQF95_RS24860 (GQF95_24830)	81366..82199	+	834	WP_226149389.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93419	93419

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T145328 WP_000162415.1 NZ_CP047013:76772-77074 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T145328 NZ_CP062358:1153427-1153615 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13068.01 Da
• Isoelectric Point: 6.4754

>AT145328 WP_032220986.1 NZ_CP047013:77145-77483 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNMNKKAA

Nucleotide

• Length: 339 bp

>AT145328 NZ_CP062358:1153138-1153398 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3