Detailed information of TA system TA145323

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51256..51520
• Replicon: plasmid pCMY-2
• Accession: NZ_CP047012
• Organism: Escherichia coli strain 19-5

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: GQF95_RS24135
• Protein ID: WP_001303307.1
• Coordinates: 51368..51520 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	51256..51318 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF95_RS24120 (46495)	46495..48786	-	2292	WP_001289271.1	F-type conjugative transfer protein TrbC	-
GQF95_RS24125 (48779)	48779..49849	-	1071	WP_000151575.1	IncI1-type conjugal transfer protein TrbB	-
GQF95_RS24130 (49868)	49868..51076	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (51256)	51256..51318	-	63	NuclAT_0	-	Antitoxin
- (51256)	51256..51318	-	63	NuclAT_0	-	Antitoxin
- (51256)	51256..51318	-	63	NuclAT_0	-	Antitoxin
- (51256)	51256..51318	-	63	NuclAT_0	-	Antitoxin
GQF95_RS24135 (51368)	51368..51520	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
GQF95_RS24140 (51592)	51592..51843	-	252	WP_001291965.1	hypothetical protein	-
GQF95_RS24145 (52204)	52204..52986	-	783	WP_001300609.1	IS21-like element IS100kyp family helper ATPase IstB	-
GQF95_RS24150 (52983)	52983..54005	-	1023	WP_001572805.1	IS21-like element IS100 family transposase	-
- (54189)	54189..54240	-	52	NuclAT_1	-	-
- (54189)	54189..54240	-	52	NuclAT_1	-	-
- (54189)	54189..54240	-	52	NuclAT_1	-	-
- (54189)	54189..54240	-	52	NuclAT_1	-	-
GQF95_RS24155 (54726)	54726..54902	-	177	WP_001054900.1	hypothetical protein	-
GQF95_RS24160 (55294)	55294..55503	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
GQF95_RS24165 (55575)	55575..56237	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..101644	101644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T145323 WP_001303307.1 NZ_CP047012:51368-51520 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T145323 NZ_CP062356:2647708-2648070 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 63 bp

>AT145323 NZ_CP047012:c51318-51256 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin