Detailed information of TA system TA145315

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110177..110603
• Replicon: plasmid pfloR
• Accession: NZ_CP047011
• Organism: Escherichia coli strain 19-5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: GQF95_RS23545
• Protein ID: WP_001372321.1
• Coordinates: 110177..110302 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	110379..110603 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF95_RS23510 (105200)	105200..105427	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
GQF95_RS23515 (105564)	105564..106235	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
GQF95_RS23520 (106429)	106429..106812	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
GQF95_RS23525 (107135)	107135..107737	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
GQF95_RS23530 (108034)	108034..108855	-	822	WP_001234445.1	DUF932 domain-containing protein	-
GQF95_RS23535 (108966)	108966..109262	-	297	WP_001272251.1	hypothetical protein	-
GQF95_RS23540 (109562)	109562..109858	+	297	Protein_137	hypothetical protein	-
GQF95_RS23545 (110177)	110177..110302	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
GQF95_RS26075 (110244)	110244..110393	-	150	Protein_139	plasmid maintenance protein Mok	-
GQF95_RS23550 (110415)	110415..110594	+	180	WP_001309233.1	hypothetical protein	-
- (110379)	110379..110603	-	225	NuclAT_0	-	Antitoxin
- (110379)	110379..110603	-	225	NuclAT_0	-	Antitoxin
- (110379)	110379..110603	-	225	NuclAT_0	-	Antitoxin
- (110379)	110379..110603	-	225	NuclAT_0	-	Antitoxin
GQF95_RS23555 (110572)	110572..111334	-	763	Protein_141	plasmid SOS inhibition protein A	-
GQF95_RS23560 (111331)	111331..111765	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GQF95_RS23565 (111834)	111834..113857	-	2024	Protein_143	ParB/RepB/Spo0J family partition protein	-
GQF95_RS23570 (113918)	113918..114151	-	234	WP_000006003.1	DUF905 family protein	-
GQF95_RS23575 (114209)	114209..114736	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
GQF95_RS26080 (115038)	115038..115493	+	456	Protein_146	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / mph(A) / aac(3)-IId / blaTEM-1B / sul3 / aph(3')-Ia / tet(A) / sul2 / floR / erm(42) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..161471	161471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T145315 WP_001372321.1 NZ_CP047011:c110302-110177 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T145315 NZ_CP062356:121643-121795 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT145315 NZ_CP047011:c110603-110379 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin