Detailed information of TA system TA145290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 809815..810509
• Replicon: chromosome
• Accession: NZ_CP047010
• Organism: Escherichia coli strain 19-5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GQF95_RS04015
• Protein ID: WP_001263493.1
• Coordinates: 810111..810509 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GQF95_RS04010
• Protein ID: WP_000554757.1
• Coordinates: 809815..810108 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF95_RS03990 (805447)	805447..805944	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
GQF95_RS03995 (806168)	806168..807880	-	1713	Protein_781	flagellar biosynthesis protein FlhA	-
GQF95_RS04000 (807852)	807852..808637	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
GQF95_RS04005 (808708)	808708..809763	+	1056	WP_063501914.1	DNA polymerase IV	-
GQF95_RS04010 (809815)	809815..810108	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GQF95_RS04015 (810111)	810111..810509	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GQF95_RS04020 (810519)	810519..810971	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GQF95_RS04025 (811217)	811217..811420	+	204	Protein_787	RNA ligase RtcB family protein	-
GQF95_RS04030 (811419)	811419..811940	+	522	Protein_788	peptide chain release factor H	-
GQF95_RS04035 (811997)	811997..813454	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
GQF95_RS04040 (813715)	813715..814173	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (814769)	814769..814849	+	81	NuclAT_11	-	-
- (814769)	814769..814849	+	81	NuclAT_11	-	-
- (814769)	814769..814849	+	81	NuclAT_11	-	-
- (814769)	814769..814849	+	81	NuclAT_11	-	-
GQF95_RS04045 (814265)	814265..815509	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	792169..839048	46879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T145290 WP_001263493.1 NZ_CP047010:810111-810509 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T145290 NZ_CP062350:2577635-2577739 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT145290 WP_000554757.1 NZ_CP047010:809815-810108 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT145290 NZ_CP062350:c2577897-2577734 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6