Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4123079..4123620 Replicon chromosome
Accession NZ_CP047002
Organism Escherichia coli strain J-8

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag GQF94_RS19645 Protein ID WP_000615982.1
Coordinates 4123079..4123357 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GQF94_RS19650 Protein ID WP_000729704.1
Coordinates 4123360..4123620 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF94_RS19630 (4120661) 4120661..4121239 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
GQF94_RS19635 (4121445) 4121445..4122212 + 768 WP_000333380.1 class II glutamine amidotransferase -
GQF94_RS19640 (4122183) 4122183..4122923 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
GQF94_RS19645 (4123079) 4123079..4123357 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GQF94_RS19650 (4123360) 4123360..4123620 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GQF94_RS19655 (4123806) 4123806..4124579 + 774 WP_000543899.1 C40 family peptidase -
GQF94_RS19660 (4124636) 4124636..4124992 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GQF94_RS19665 (4124985) 4124985..4125263 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GQF94_RS19670 (4125368) 4125368..4127080 - 1713 Protein_3868 flagellar biosynthesis protein FlhA -
GQF94_RS19675 (4127052) 4127052..4127837 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T145275 WP_000615982.1 NZ_CP047002:c4123357-4123079 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T145275 NZ_CP062348:c1754895-1754719 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT145275 WP_000729704.1 NZ_CP047002:c4123620-4123360 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT145275 NZ_CP062348:1754588-1754729 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References