Detailed information of TA system TA145272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3267868..3268470
• Replicon: chromosome
• Accession: NZ_CP047002
• Organism: Escherichia coli strain J-8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GQF94_RS15725
• Protein ID: WP_000897305.1
• Coordinates: 3267868..3268179 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GQF94_RS15730
• Protein ID: WP_000356397.1
• Coordinates: 3268180..3268470 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF94_RS15695 (3262898)	3262898..3263683	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
GQF94_RS15700 (3263782)	3263782..3264381	+	600	WP_001295269.1	glucose-1-phosphatase	-
GQF94_RS15705 (3264375)	3264375..3265247	+	873	WP_000920762.1	virulence factor BrkB family protein	-
GQF94_RS15710 (3265244)	3265244..3265681	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
GQF94_RS15715 (3265726)	3265726..3266667	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GQF94_RS15720 (3266731)	3266731..3267639	-	909	WP_001162704.1	alpha/beta hydrolase	-
GQF94_RS15725 (3267868)	3267868..3268179	+	312	WP_000897305.1	hypothetical protein	Toxin
GQF94_RS15730 (3268180)	3268180..3268470	+	291	WP_000356397.1	NadS family protein	Antitoxin
GQF94_RS15735 (3269056)	3269056..3269274	+	219	WP_001611167.1	CopG family transcriptional regulator	-
GQF94_RS15740 (3269493)	3269493..3269735	+	243	WP_001086388.1	protein YiiF	-
GQF94_RS15745 (3270065)	3270065..3270994	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GQF94_RS15750 (3270991)	3270991..3271626	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GQF94_RS15755 (3271623)	3271623..3272525	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T145272 WP_000897305.1 NZ_CP047002:3267868-3268179 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T145272 NZ_CP062348:c526525-526337 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT145272 WP_000356397.1 NZ_CP047002:3268180-3268470 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT145272 NZ_CP062348:c526814-526554 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7