Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2432348..2433147 Replicon chromosome
Accession NZ_CP047002
Organism Escherichia coli strain J-8

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag GQF94_RS11695 Protein ID WP_000347266.1
Coordinates 2432683..2433147 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GQF94_RS11690 Protein ID WP_001307405.1
Coordinates 2432348..2432683 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF94_RS11675 (2428133) 2428133..2428903 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GQF94_RS11680 (2428919) 2428919..2430253 - 1335 WP_000599640.1 galactarate/glucarate/glycerate transporter GarP -
GQF94_RS11685 (2430628) 2430628..2432199 + 1572 WP_001273741.1 galactarate dehydratase -
GQF94_RS11690 (2432348) 2432348..2432683 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GQF94_RS11695 (2432683) 2432683..2433147 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GQF94_RS11700 (2433202) 2433202..2434011 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GQF94_RS11705 (2434260) 2434260..2435540 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
GQF94_RS11710 (2435563) 2435563..2436036 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GQF94_RS11715 (2436047) 2436047..2436826 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
GQF94_RS11720 (2436816) 2436816..2437694 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
GQF94_RS11725 (2437712) 2437712..2438146 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2421692..2433147 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T145269 WP_000347266.1 NZ_CP047002:2432683-2433147 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T145269 NZ_CP062346:2709742-2709951 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACGCCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT145269 WP_001307405.1 NZ_CP047002:2432348-2432683 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT145269 NZ_CP062346:2709437-2709730 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAAGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References