Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1225481..1225983 Replicon chromosome
Accession NZ_CP047002
Organism Escherichia coli strain J-8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GQF94_RS06055 Protein ID WP_000767829.1
Coordinates 1225481..1225735 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A2S1Y4D6
Locus tag GQF94_RS06060 Protein ID WP_001259254.1
Coordinates 1225732..1225983 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF94_RS06025 (1220496) 1220496..1220723 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
GQF94_RS06030 (1220737) 1220737..1221795 - 1059 WP_024193414.1 thiosulfate utilization transporter TsuA/YeeE -
GQF94_RS06035 (1221974) 1221974..1223332 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GQF94_RS06040 (1223322) 1223322..1223384 - 63 WP_010723108.1 membrane protein YoeI -
GQF94_RS06045 (1223599) 1223599..1224528 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
GQF94_RS06050 (1224574) 1224574..1225398 - 825 WP_000754737.1 SDR family oxidoreductase -
GQF94_RS06055 (1225481) 1225481..1225735 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GQF94_RS06060 (1225732) 1225732..1225983 - 252 WP_001259254.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GQF94_RS06065 (1226266) 1226266..1226316 + 51 WP_001364200.1 his operon leader peptide -
GQF94_RS06070 (1226462) 1226462..1227361 + 900 WP_072645750.1 ATP phosphoribosyltransferase -
GQF94_RS06075 (1227367) 1227367..1228671 + 1305 WP_001345878.1 histidinol dehydrogenase -
GQF94_RS06080 (1228668) 1228668..1229738 + 1071 WP_072645751.1 histidinol-phosphate transaminase -
GQF94_RS06085 (1229738) 1229738..1230805 + 1068 WP_000080109.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T145264 WP_000767829.1 NZ_CP047002:c1225735-1225481 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T145264 NZ_CP062346:c1726047-1725796 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATAGAACAATCCGTTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACATATATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTTATCTTGATGAAGCAGTAGGGATTATGACGAGTCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9292.57 Da        Isoelectric Point: 4.5822

>AT145264 WP_001259254.1 NZ_CP047002:c1225983-1225732 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGIGTEKD
IIE

Download         Length: 252 bp

>AT145264 NZ_CP062346:c1725806-1725624 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATATACATTACAAGTTATCTGACGGCTCTATTATCAGTCTCATAGACTTTGTTATTAAACCAATTCATTTAATCA
AGGAGGCACAGGAAAATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A2S1Y4D6

References