Detailed information of TA system TA145239

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39801..40070
• Replicon: plasmid pKPC2_020130
• Accession: NZ_CP046963
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020130

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: CLQ86_RS27230
• Protein ID: WP_001372321.1
• Coordinates: 39945..40070 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	39801..39866 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ86_RS27200	35511..36038	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CLQ86_RS27205	36096..36329	+	234	WP_000006003.1	DUF905 family protein	-
CLQ86_RS27210	36390..38413	+	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
CLQ86_RS27215	38482..38916	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CLQ86_RS27220	38913..39632	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	39644..39868	+	225	NuclAT_0	-	-
-	39644..39868	+	225	NuclAT_0	-	-
-	39644..39868	+	225	NuclAT_0	-	-
-	39644..39868	+	225	NuclAT_0	-	-
-	39801..39866	+	66	-	-	Antitoxin
CLQ86_RS27225	39854..40003	+	150	Protein_50	plasmid maintenance protein Mok	-
CLQ86_RS27230	39945..40070	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
CLQ86_RS27235	40389..40685	-	297	Protein_52	hypothetical protein	-
CLQ86_RS27240	40985..41281	+	297	WP_001272251.1	hypothetical protein	-
CLQ86_RS27245	41392..42213	+	822	WP_001234445.1	DUF932 domain-containing protein	-
CLQ86_RS27250	42510..43157	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
CLQ86_RS27255	43434..43817	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
CLQ86_RS27260	44008..44694	+	687	WP_015059009.1	PAS domain-containing protein	-
CLQ86_RS27265	44788..45015	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..152833	152833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T145239 WP_001372321.1 NZ_CP046963:39945-40070 [Klebsiella pneumoniae subsp. pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T145239 NZ_CP062340:2694604-2694966 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT145239 NZ_CP046963:39801-39866 [Klebsiella pneumoniae subsp. pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin