Detailed information of TA system TA145215

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67732..67985
• Replicon: plasmid pKPC2_015247
• Accession: NZ_CP046958
• Organism: Klebsiella pneumoniae strain WCHKP015247

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C6M24_RS27235
• Protein ID: WP_001312851.1
• Coordinates: 67732..67881 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	67926..67985 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M24_RS27200 (63091)	63091..63506	-	416	Protein_73	IS1-like element IS1B family transposase	-
C6M24_RS27205 (63755)	63755..64156	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
C6M24_RS27210 (64089)	64089..64346	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
C6M24_RS27215 (64439)	64439..65092	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
C6M24_RS27220 (66031)	66031..66888	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
C6M24_RS27225 (66881)	66881..66955	-	75	WP_001375168.1	RepA leader peptide Tap	-
C6M24_RS27230 (67200)	67200..67448	-	249	WP_000083837.1	replication regulatory protein RepA	-
C6M24_RS27235 (67732)	67732..67881	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (67926)	67926..67985	+	60	NuclAT_0	-	Antitoxin
- (67926)	67926..67985	+	60	NuclAT_0	-	Antitoxin
- (67926)	67926..67985	+	60	NuclAT_0	-	Antitoxin
- (67926)	67926..67985	+	60	NuclAT_0	-	Antitoxin
C6M24_RS27240 (68186)	68186..68518	-	333	WP_152916585.1	hypothetical protein	-
C6M24_RS27245 (68580)	68580..69179	-	600	WP_032083981.1	PIN domain-containing protein	-
C6M24_RS27250 (69565)	69565..69765	-	201	WP_015059022.1	hypothetical protein	-
C6M24_RS27255 (69897)	69897..70457	-	561	WP_000139328.1	fertility inhibition protein FinO	-
C6M24_RS27260 (70512)	70512..71258	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C6M24_RS27265 (71278)	71278..71478	-	201	WP_072354025.1	hypothetical protein	-
C6M24_RS27270 (71503)	71503..72207	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / catA2 / blaCTX-M-65 / blaTEM-1C / tet(A)	-	1..97576	97576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T145215 WP_001312851.1 NZ_CP046958:c67881-67732 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T145215 NZ_CP062336:c2061626-2061360 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT145215 NZ_CP046958:67926-67985 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin