Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 356945..357591 Replicon chromosome
Accession NZ_CP046957
Organism Klebsiella pneumoniae strain WCHKP015247

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag C6M24_RS01660 Protein ID WP_002920560.1
Coordinates 356945..357292 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag C6M24_RS01665 Protein ID WP_002920557.1
Coordinates 357292..357591 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M24_RS01650 (C6M24_01630) 352871..354304 + 1434 WP_002920564.1 glycogen synthase GlgA -
C6M24_RS01655 (C6M24_01635) 354322..356769 + 2448 WP_002920561.1 glycogen phosphorylase -
C6M24_RS01660 (C6M24_01640) 356945..357292 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6M24_RS01665 (C6M24_01645) 357292..357591 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
C6M24_RS01670 (C6M24_01650) 357654..359162 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
C6M24_RS01675 (C6M24_01655) 359367..359696 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
C6M24_RS01680 (C6M24_01660) 359747..360577 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
C6M24_RS01685 (C6M24_01665) 360627..361385 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T145198 WP_002920560.1 NZ_CP046957:356945-357292 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T145198 NZ_CP062334:18831-19007 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT145198 WP_002920557.1 NZ_CP046957:357292-357591 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT145198 NZ_CP062334:c19169-18995 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGATGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References