Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 330756..331342 Replicon chromosome
Accession NZ_CP046957
Organism Klebsiella pneumoniae strain WCHKP015247

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag C6M24_RS01550 Protein ID WP_002920800.1
Coordinates 330974..331342 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag C6M24_RS01545 Protein ID WP_002920802.1
Coordinates 330756..330977 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M24_RS01525 (C6M24_01505) 326913..327839 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
C6M24_RS01530 (C6M24_01510) 327836..329113 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
C6M24_RS01535 (C6M24_01515) 329110..329877 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
C6M24_RS01540 (C6M24_01520) 329879..330592 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
C6M24_RS01545 (C6M24_01525) 330756..330977 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C6M24_RS01550 (C6M24_01530) 330974..331342 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
C6M24_RS01555 (C6M24_01535) 331615..332931 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
C6M24_RS01560 (C6M24_01540) 333038..333925 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
C6M24_RS01565 (C6M24_01545) 333922..334767 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
C6M24_RS01570 (C6M24_01550) 334769..335839 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327836..336576 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T145197 WP_002920800.1 NZ_CP046957:330974-331342 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T145197 NZ_CP062331:2745191-2745553 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT145197 WP_002920802.1 NZ_CP046957:330756-330977 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT145197 NZ_CP062331:2745024-2745194 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References