Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3936783..3937380 Replicon chromosome
Accession NZ_CP046949
Organism Klebsiella pneumoniae strain BD_DM_914

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag GQY16_RS19110 Protein ID WP_004142563.1
Coordinates 3937063..3937380 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag GQY16_RS19105 Protein ID WP_004142561.1
Coordinates 3936783..3937070 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQY16_RS19075 3932863..3933111 + 249 WP_020325243.1 DUF1158 domain-containing protein -
GQY16_RS19080 3933129..3933470 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
GQY16_RS19085 3933501..3934616 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
GQY16_RS19090 3934796..3935377 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
GQY16_RS19095 3935377..3935745 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
GQY16_RS19100 3935865..3936518 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
GQY16_RS19105 3936783..3937070 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
GQY16_RS19110 3937063..3937380 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GQY16_RS19115 3937565..3938608 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
GQY16_RS19120 3939278..3940144 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
GQY16_RS19125 3940253..3941680 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T145190 WP_004142563.1 NZ_CP046949:c3937380-3937063 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T145190 NZ_CP062331:c180060-179965 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT145190 WP_004142561.1 NZ_CP046949:c3937070-3936783 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT145190 NZ_CP062331:179878-179937 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References