Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3892623..3893220 Replicon chromosome
Accession NZ_CP046939
Organism Klebsiella pneumoniae strain BD_DM_697

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag GQY17_RS18795 Protein ID WP_004142563.1
Coordinates 3892903..3893220 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag GQY17_RS18790 Protein ID WP_004142561.1
Coordinates 3892623..3892910 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQY17_RS18760 3888703..3888951 + 249 WP_020325243.1 DUF1158 domain-containing protein -
GQY17_RS18765 3888969..3889310 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
GQY17_RS18770 3889341..3890456 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
GQY17_RS18775 3890636..3891217 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
GQY17_RS18780 3891217..3891585 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
GQY17_RS18785 3891705..3892358 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
GQY17_RS18790 3892623..3892910 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
GQY17_RS18795 3892903..3893220 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GQY17_RS18800 3893405..3894448 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
GQY17_RS18805 3895118..3895984 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
GQY17_RS18810 3896093..3897520 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T145170 WP_004142563.1 NZ_CP046939:c3893220-3892903 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T145170 NZ_CP062326:20057-20233 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT145170 WP_004142561.1 NZ_CP046939:c3892910-3892623 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT145170 NZ_CP062326:c20395-20221 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References