Detailed information of TA system TA145136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3705979..3706637
• Replicon: chromosome
• Accession: NZ_CP046904
• Organism: Massilia flava strain DSM 26639

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GO485_RS15705
• Protein ID: WP_145880604.1
• Coordinates: 3706290..3706637 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GO485_RS15700
• Protein ID: WP_145880602.1
• Coordinates: 3705979..3706293 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO485_RS15685	3703700..3704170	-	471	WP_145880595.1	hypothetical protein	-
GO485_RS15690	3704230..3705507	+	1278	WP_145880597.1	SDR family oxidoreductase	-
GO485_RS15695	3705507..3705971	+	465	WP_145880599.1	DUF2269 domain-containing protein	-
GO485_RS15700	3705979..3706293	-	315	WP_145880602.1	transcriptional regulator	Antitoxin
GO485_RS15705	3706290..3706637	-	348	WP_145880604.1	toxin	Toxin
GO485_RS15710	3706965..3707618	+	654	WP_145880606.1	hydrolase	-
GO485_RS15715	3707596..3708210	-	615	WP_145880707.1	YdeI/OmpD-associated family protein	-
GO485_RS15720	3708210..3708641	-	432	WP_145880608.1	SRPBCC family protein	-
GO485_RS15725	3708692..3709630	+	939	WP_145880611.1	LysR family transcriptional regulator	-
GO485_RS15730	3709722..3710942	+	1221	WP_145880613.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-89)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13502.36 Da
• Isoelectric Point: 8.3800

>T145136 WP_145880604.1 NZ_CP046904:c3706637-3706290 [Massilia flava]
MKATFIELPPFERVRDDYFDDESFKDLQNELMKNPEAGDVIPGAGGLRKIRYGDERRGKGKRGGLRVIYFWKDADDQFWL
FTVYGKDEAADLTPEQRKMLKQRLELEIKARRATQ

Nucleotide

• Length: 348 bp

>T145136 NZ_CP062318:2704133-2704450 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11885.77 Da
• Isoelectric Point: 8.6178

>AT145136 WP_145880602.1 NZ_CP046904:c3706293-3705979 [Massilia flava]
MTKRDVFADLMEGFDALEQARKGKLTLRTTKVEYQSPPEMTPEQVRAVREKLHVSQPVFARQLRTELRTIKNWEQGISKP
NAQAAILLSLIDRDPEILDLIAAL

Nucleotide

• Length: 315 bp

>AT145136 NZ_CP062318:2703921-2704130 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCCTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA