Detailed information of TA system TA145055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1036455..1037053
• Replicon: chromosome
• Accession: NZ_CP046840
• Organism: Vibrio cholerae strain F9993

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q8KQX9
• Locus tag: GPY07_RS04630
• Protein ID: WP_000118545.1
• Coordinates: 1036455..1036778 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GPY07_RS04635
• Protein ID: WP_000058216.1
• Coordinates: 1036775..1037053 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY07_RS04600	1032168..1032554	-	387	WP_000180225.1	hypothetical protein	-
GPY07_RS04605	1032551..1033123	-	573	WP_139057167.1	type IV conjugative transfer system lipoprotein TraV	-
GPY07_RS04610	1033198..1034487	-	1290	WP_001883765.1	TraB/VirB10 family protein	-
GPY07_RS04615	1034490..1035386	-	897	WP_032467354.1	type-F conjugative transfer system secretin TraK	-
GPY07_RS04620	1035370..1035996	-	627	WP_000667169.1	hypothetical protein	-
GPY07_RS04625	1035993..1036274	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
GPY07_RS04630	1036455..1036778	+	324	WP_000118545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPY07_RS04635	1036775..1037053	+	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
GPY07_RS04640	1037079..1037714	-	636	WP_000033753.1	DUF4400 domain-containing protein	-
GPY07_RS04645	1037701..1038261	-	561	WP_000546510.1	hypothetical protein	-
GPY07_RS04650	1038271..1040091	-	1821	WP_000661887.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul2 / aph(3'')-Ib / aph(6)-Id / floR / dfrA18	-	944860..1092902	148042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12267.26 Da
• Isoelectric Point: 9.4705

>T145055 WP_000118545.1 NZ_CP046840:1036455-1036778 [Vibrio cholerae]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGNHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T145055 NZ_CP062251:4774068-4774421 [Pseudomonas sp. IzPS59]
ATGAGTACAAGGAAGCCACTCCTGTGGGTTAGCAGTAGCAAGAAAGATCTCAAGCAGATGCCCACAGATGTACAGGATGT
TTTTGGTTACGCACTGGATTTGGCCCAAAGCGGTCTCAGACATCCGGATGCAAAACCTCTGAAGGGGTTTGGAGGCGCGG
GTGTCCTTGAGTTGATTGAGGACTTTGATACGAATACCTACCGTGCCGTTTACACAGTCCGTTTTGGCAATGCGATTTAC
GTCCTGCATTGTTTTCAGAAAAAGTCGACCGCAGGTATCAAAACCGCGCAATGCGATATCGACCTGATCCGAAAGAGACT
TCAAGCCGCTCAGGACCACGCGAAAGGAACATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT145055 WP_000058216.1 NZ_CP046840:1036775-1037053 [Vibrio cholerae]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT145055 NZ_CP062251:4774424-4774744 [Pseudomonas sp. IzPS59]
ATGAATGAAATCGAAGAAGGCAGCGGAAACGTCTATCAAGACCTTGGCTTACCAAATGCCAGTGAAATGCAGGTCAAGTC
ACAGCTAGCCGCAAAGATCGGCGAAATCATCAAAGCGCGTCATCTCACTCAATCTCAAGCATCTGAAATCCTGGGGCTAT
CACAACCCAAGCTGTCCGAAATGCTGAGAGGAAAATTCCGCGGCATCAGTGAAGCCAAGATGATGGAATGCCTATCCCAC
CTCGGCCGAGATGTGCAGATTGTGGTCAAGCCGGCCCCCCGCTCCCGCAAAGAGGGCCGAATTGAAGTGGTGTTTGCGTA
A

Structures

Toxin

• AlphaFold DB: A0A0X1L383