Detailed information of TA system TA145051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2287325..2287923
• Replicon: chromosome
• Accession: NZ_CP046839
• Organism: Vibrio cholerae strain 2011EL-1271

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Z7VLH5
• Locus tag: GPY01_RS15005
• Protein ID: WP_054103799.1
• Coordinates: 2287600..2287923 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GPY01_RS15000
• Protein ID: WP_000058216.1
• Coordinates: 2287325..2287603 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY01_RS14985	2284287..2286107	+	1821	WP_123161585.1	conjugative transfer system coupling protein TraD	-
GPY01_RS14990	2286117..2286677	+	561	WP_123161586.1	conjugative transfer protein	-
GPY01_RS14995	2286664..2287299	+	636	WP_123161587.1	DUF4400 domain-containing protein	-
GPY01_RS15000	2287325..2287603	-	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
GPY01_RS15005	2287600..2287923	-	324	WP_054103799.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPY01_RS15010	2288104..2288385	+	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
GPY01_RS15015	2288382..2289008	+	627	WP_000667170.1	hypothetical protein	-
GPY01_RS15020	2288992..2289888	+	897	WP_123161676.1	type-F conjugative transfer system secretin TraK	-
GPY01_RS15025	2289891..2291180	+	1290	WP_123161588.1	TraB/VirB10 family protein	-
GPY01_RS15030	2291255..2291827	+	573	WP_148039504.1	type IV conjugative transfer system lipoprotein TraV	-
GPY01_RS15035	2291824..2292210	+	387	WP_123161589.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12295.35 Da
• Isoelectric Point: 9.7658

>T145051 WP_054103799.1 NZ_CP046839:c2287923-2287600 [Vibrio cholerae]
MSWKIDFYDGVEEQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T145051 NZ_CP062251:c1803945-1803370 [Pseudomonas sp. IzPS59]
GTGAGGCATTCCTCTTTTACCGCTTTGTACGATGCATGCGTCTTGTACCCCGCCCCCTTGAGAGACTTCCTCATGTGGCT
TGCGCTCTCGGGACGATTTCGGGCGCGATGGACCCAGGAGATTCACTGCGAATGGAAACGCAATCTGCTGAAGAACCGCC
CGGACCTGACGATGGAGCAGCTGGATCGCACCTCAAACCTTATGGATCAAGCAATACCCGACGCCTGCGTCCATGACTAT
GAAGACCTGATTGCCGGCCTCACCTTGCCCGACTGCGATGATCGTCACGTTCTGGCAGCGGCCATCCGCTGCGGCGCAAG
CGTGATAGTGACGTTCAACCAGAAAGACTTTCCCTGCAGATCGCTGGAGCCTTTCGGCATCGAAGCGCAGCATCCGGACG
AGTTCGTCGACAACCTCTTGGGGCTTGATCCGGCAGCAGTGGTTGCAGCGGCCCAAAAGCAGCGTCGGCAGCTGAAAATG
CCTGCCATGGACGTCGAACCCTTTCTTGATCTACTGCAACGACAAGGCCTGACTAAATCGGTCCAGGCACTAAGCAGCTA
TCACTCGATTCTTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT145051 WP_000058216.1 NZ_CP046839:c2287603-2287325 [Vibrio cholerae]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT145051 NZ_CP062251:c1804409-1803942 [Pseudomonas sp. IzPS59]
ATGTCGACCGTTATTCATCCCTCCATCAGCCTCCCCGTAGAACGTGAAGTCAAAGCCGCCATTGAAGGGCAGCGTGCCCT
GGCTGCTTACCTTGCGACCCATTTCGAGACCCAACACATCCAGATTTTCGACGAGCAGAATGAAGCTCATCGCGTCGAAC
TGCCCACCTCGGCCTTGCGCCTGCTGATCGACATTCTGGCGGAGCTGGCAGCCGGCAACGCGGTCCAGGTCGTGCCGGTG
CATGCTGAATTGACGACTCAGGAAGCCGCCGACCTGCTCAACGTCTCACGTCCACACCTCATAAAACTGCTGGAAAGCGG
CGAGTTGTCCTACCACAAAACAGGCAAGCACCGACGTGTGCGTTTTGCCGATCTGATGGACTATAAGACCCGGCGCACCA
CCGCCAGCGAGCAGGCAATGGCGCTTCTCGCAGAGCAAGCTCAGGCGTTAAGGACAGGTTACGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7Z7VLH5