Detailed information of TA system TA145005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 728960..729575
• Replicon: chromosome
• Accession: NZ_CP046821
• Organism: Vibrio metschnikovii strain 07-2421

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GPX85_RS03200
• Protein ID: WP_199249638.1
• Coordinates: 729288..729575 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7Y3VLD0
• Locus tag: GPX85_RS03195
• Protein ID: WP_039438324.1
• Coordinates: 728960..729277 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPX85_RS03165	725232..725564	+	333	WP_158137348.1	SEC-C domain-containing protein	-
GPX85_RS03170	725772..726062	-	291	WP_002033345.1	hypothetical protein	-
GPX85_RS03175	726743..727705	+	963	WP_158137349.1	IS110 family transposase	-
GPX85_RS03180	727739..728212	-	474	WP_158137350.1	hypothetical protein	-
GPX85_RS03185	728370..728825	-	456	WP_158116645.1	DUF2127 domain-containing protein	-
GPX85_RS03190	728853..729008	-	156	Protein_633	DUF3265 domain-containing protein	-
GPX85_RS03195	728960..729277	-	318	WP_039438324.1	HigA family addiction module antidote protein	Antitoxin
GPX85_RS03200	729288..729575	-	288	WP_199249638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPX85_RS03205	730375..730566	-	192	WP_158137352.1	DUF3265 domain-containing protein	-
GPX85_RS03210	731013..732359	+	1347	WP_158137016.1	group II intron reverse transcriptase/maturase	-
GPX85_RS03215	732445..733193	-	749	Protein_638	PhzF family phenazine biosynthesis protein	-
GPX85_RS03220	733239..733430	-	192	WP_158137353.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	725772..821261	95489
	-	flank	IS/Tn	-	-	726743..727705	962
	-	inside	Integron	-	katB / cqsA	3..822451	822448
	-	inside	Integron	-	-	727739..729798	2059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11305.89 Da
• Isoelectric Point: 6.7187

>T145005 WP_199249638.1 NZ_CP046821:c729575-729288 [Vibrio metschnikovii]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQGESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNAYLDPHKY

Nucleotide

• Length: 288 bp

>T145005 NZ_CP062249:c1918663-1918560 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11719.66 Da
• Isoelectric Point: 10.4711

>AT145005 WP_039438324.1 NZ_CP046821:c729277-728960 [Vibrio metschnikovii]
MRKTKRRPVSVGEMLKLEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT145005 NZ_CP062249:1918522-1918793 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A7Y3VLD0