Detailed information of TA system TA144934

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 2410561..2411194
• Replicon: chromosome
• Accession: NZ_CP046785
• Organism: Vibrio parahaemolyticus strain 2013V-1136

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A347UQS4
• Locus tag: GPY45_RS12035
• Protein ID: WP_012600340.1
• Coordinates: 2410561..2410893 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GPY45_RS12040
• Protein ID: WP_017049537.1
• Coordinates: 2410880..2411194 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY45_RS11970	2405573..2405692	+	120	WP_103181466.1	DUF3265 domain-containing protein	-
GPY45_RS11975	2405780..2406325	+	546	WP_005433704.1	hypothetical protein	-
GPY45_RS11980	2406478..2407143	+	666	WP_089202955.1	hypothetical protein	-
GPY45_RS11985	2407164..2407256	+	93	WP_089202954.1	DUF3265 domain-containing protein	-
GPY45_RS11990	2407306..2407947	+	642	WP_089202953.1	hypothetical protein	-
GPY45_RS11995	2407967..2408059	+	93	WP_072612521.1	DUF3265 domain-containing protein	-
GPY45_RS12000	2408067..2408384	-	318	WP_053300098.1	CcdB family protein	-
GPY45_RS12005	2408384..2408629	-	246	WP_089202951.1	type II toxin-antitoxin system CcdA family antitoxin	-
GPY45_RS12010	2408704..2408796	+	93	WP_089202950.1	DUF3265 domain-containing protein	-
GPY45_RS12020	2409308..2409742	+	435	WP_141106041.1	hypothetical protein	-
GPY45_RS12025	2409998..2410201	+	204	WP_190970714.1	hypothetical protein	-
GPY45_RS12030	2410369..2410458	+	90	WP_079854229.1	DUF3265 domain-containing protein	-
GPY45_RS12035	2410561..2410893	+	333	WP_012600340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPY45_RS12040	2410880..2411194	+	315	WP_017049537.1	DNA-binding transcriptional regulator	Antitoxin
GPY45_RS12045	2411209..2411301	+	93	WP_089202946.1	DUF3265 domain-containing protein	-
GPY45_RS12050	2411403..2411735	+	333	WP_089202945.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GPY45_RS12055	2411722..2412036	+	315	WP_089202944.1	DNA-binding transcriptional regulator	-
GPY45_RS12060	2412171..2412962	+	792	WP_089202943.1	hypothetical protein	-
GPY45_RS12065	2412977..2413069	+	93	WP_089202942.1	DUF3265 domain-containing protein	-
GPY45_RS12070	2413096..2413620	+	525	WP_089202941.1	GNAT family N-acetyltransferase	-
GPY45_RS12075	2413744..2414496	+	753	WP_031828262.1	DUF2971 domain-containing protein	-
GPY45_RS12080	2414511..2414603	+	93	WP_079720270.1	DUF3265 domain-containing protein	-
GPY45_RS12085	2414643..2415188	+	546	WP_089202940.1	GNAT family N-acetyltransferase	-
GPY45_RS12090	2415343..2415702	+	360	WP_089202939.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vpadF / vpadF / vxsC / virG / exsA / exsD / vscB / vscC / vscD / vscF / vscG / vscH / vscI / vscJ / vscK / vscL / vopS / vopR / vecA / vopQ / vscU / vscT / vscS / vscR / vscQ / vscP / vscO / vscN / vopN / tyeA/vcr1 / sycN/vcr2 / vscX / vscY / vcrD / vcrR / vcrG / vcrV / vcrH / vopB / vopD / VP1611	2322112..2711902	389790
	-	inside	Integron	-	-	2397971..2490093	92122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (24-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12906.76 Da
• Isoelectric Point: 10.0960

>T144934 WP_012600340.1 NZ_CP046785:2410561-2410893 [Vibrio parahaemolyticus]
MKSVFVESTIFEKYRNEYLSDDEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T144934 NZ_CP062246:3270696-3271160 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11704.17 Da
• Isoelectric Point: 6.3290

>AT144934 WP_017049537.1 NZ_CP046785:2410880-2411194 [Vibrio parahaemolyticus]
MSNRDLFAELSSALVEAKEHSEGKLTLKTHQVNDISELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT144934 NZ_CP062246:3270361-3270696 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A347UQS4