Detailed information of TA system TA144920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 117877..118534
• Replicon: plasmid unnamed2
• Accession: NZ_CP046773
• Organism: Vibrio alginolyticus strain 2014V-1011

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: GPY59_RS25180
• Protein ID: WP_000270043.1
• Coordinates: 118184..118534 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GPY59_RS25175
• Protein ID: WP_000124640.1
• Coordinates: 117877..118179 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPY59_RS25130	113502..113930	+	429	WP_000591074.1	hypothetical protein	-
GPY59_RS25135	113987..114346	+	360	WP_000422768.1	hypothetical protein	-
GPY59_RS25140	114346..114792	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
GPY59_RS25145	114789..115307	+	519	WP_000210756.1	nitrite reductase	-
GPY59_RS25150	115307..115537	+	231	WP_000972663.1	hypothetical protein	-
GPY59_RS25155	115524..116381	+	858	WP_001167032.1	hypothetical protein	-
GPY59_RS25160	116612..117139	+	528	WP_001236377.1	thermonuclease family protein	-
GPY59_RS25165	117197..117469	+	273	WP_001043047.1	HU family DNA-binding protein	-
GPY59_RS25170	117557..117850	+	294	WP_001239997.1	chromosome segregation protein ParM	-
GPY59_RS25175	117877..118179	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
GPY59_RS25180	118184..118534	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPY59_RS25185	118697..119245	+	549	WP_001061574.1	transcriptional regulator	-
GPY59_RS25190	119586..119780	+	195	WP_000343597.1	hypothetical protein	-
GPY59_RS25195	119791..120162	+	372	WP_000516916.1	hypothetical protein	-
GPY59_RS25200	120155..120625	+	471	WP_001281821.1	hypothetical protein	-
GPY59_RS25205	120640..120975	-	336	WP_000683476.1	hypothetical protein	-
GPY59_RS25210	121072..121560	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
GPY59_RS25215	121563..122060	+	498	WP_000062185.1	hypothetical protein	-
GPY59_RS25220	122326..123030	+	705	WP_001067834.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3')-Ia / sul1 / qacE / dfrA15	-	1..131705	131705

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T144920 WP_000270043.1 NZ_CP046773:c118534-118184 [Vibrio alginolyticus]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T144920 NZ_CP062246:c1408184-1407990 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT144920 WP_000124640.1 NZ_CP046773:c118179-117877 [Vibrio alginolyticus]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT144920 NZ_CP062246:1407813-1407991 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9