Detailed information of TA system TA144883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 713214..713829
• Replicon: chromosome
• Accession: NZ_CP046743
• Organism: Vibrio cholerae strain 3569-08

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: GPW72_RS03870
• Protein ID: WP_001162670.1
• Coordinates: 713542..713829 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GPW72_RS03865
• Protein ID: WP_001232701.1
• Coordinates: 713214..713531 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW72_RS03830	708726..709625	-	900	WP_001887493.1	hypothetical protein	-
GPW72_RS03835	709772..710629	-	858	WP_001894914.1	DUF2806 domain-containing protein	-
GPW72_RS03840	710853..711083	+	231	WP_000838533.1	PAS factor family protein	-
GPW72_RS03845	711325..711504	-	180	WP_016711064.1	DUF645 family protein	-
GPW72_RS03850	711800..712564	-	765	WP_158149428.1	ATP-binding protein	-
GPW72_RS19250	712779..712874	-	96	WP_001907607.1	DUF645 family protein	-
GPW72_RS19255	713107..713217	-	111	WP_172837665.1	DUF3265 domain-containing protein	-
GPW72_RS03865	713214..713531	-	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
GPW72_RS03870	713542..713829	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPW72_RS03875	714110..714346	-	237	WP_000868090.1	DUF645 family protein	-
GPW72_RS03880	714597..715010	-	414	WP_000049417.1	VOC family protein	-
GPW72_RS03885	715193..715450	-	258	WP_080379067.1	hypothetical protein	-
GPW72_RS03890	716239..717201	+	963	WP_000841995.1	integron integrase IntIA	-
GPW72_RS03895	717283..717636	-	354	WP_001138366.1	50S ribosomal protein L20	-
GPW72_RS03900	717678..717872	-	195	WP_001885060.1	50S ribosomal protein L35	-
GPW72_RS03905	717976..718527	-	552	WP_001894072.1	translation initiation factor IF-3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	575627..724145	148518
	-	inside	Integron	-	-	575627..717201	141574

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T144883 WP_001162670.1 NZ_CP046743:c713829-713542 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T144883 NZ_CP062244:4438509-4438859 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT144883 WP_001232701.1 NZ_CP046743:c713531-713214 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT144883 NZ_CP062244:4438264-4438515 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36