Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 629363..629978 Replicon chromosome
Accession NZ_CP046741
Organism Vibrio cholerae strain 3523-03

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag GPW86_RS03560 Protein ID WP_001162670.1
Coordinates 629363..629650 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GPW86_RS03565 Protein ID WP_001232701.1
Coordinates 629661..629978 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPW86_RS03515 624535..625071 + 537 WP_095474066.1 hypothetical protein -
GPW86_RS03520 625220..625696 + 477 WP_102787218.1 hypothetical protein -
GPW86_RS03525 625719..626678 - 960 WP_123011654.1 IS30 family transposase -
GPW86_RS03535 627424..627705 + 282 WP_000086649.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GPW86_RS03540 627702..627989 + 288 WP_000869999.1 type II toxin-antitoxin system RelE/ParE family toxin -
GPW86_RS03545 628136..628606 - 471 WP_000784954.1 RES family NAD+ phosphorylase -
GPW86_RS03550 628606..629058 - 453 WP_000024295.1 DUF2384 domain-containing protein -
GPW86_RS03560 629363..629650 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPW86_RS03565 629661..629978 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
GPW86_RS03570 630120..630500 + 381 WP_113601670.1 MmcQ/YjbR family DNA-binding protein -
GPW86_RS03575 630536..631480 - 945 WP_038964128.1 IS110 family transposase -
GPW86_RS03580 631680..631793 + 114 WP_123011836.1 DUF3265 domain-containing protein -
GPW86_RS03585 631820..632293 + 474 WP_123011684.1 GrpB family protein -
GPW86_RS03590 632447..633451 + 1005 WP_123011685.1 LD-carboxypeptidase -
GPW86_RS03595 633542..633904 + 363 WP_032473252.1 DUF4144 domain-containing protein -
GPW86_RS03600 634066..634575 + 510 WP_000405988.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 443489..637173 193684
- flank IS/Tn - - 630536..631480 944
- inside Integron - - 573974..638391 64417


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T144864 WP_001162670.1 NZ_CP046741:629363-629650 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T144864 NZ_CP062244:c1265266-1265159 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT144864 WP_001232701.1 NZ_CP046741:629661-629978 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT144864 NZ_CP062244:1265313-1265379 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References