Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 540921..541536 Replicon chromosome
Accession NZ_CP046741
Organism Vibrio cholerae strain 3523-03

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag GPW86_RS02785 Protein ID WP_001162670.1
Coordinates 540921..541208 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GPW86_RS02790 Protein ID WP_001232701.1
Coordinates 541219..541536 (+) Length 106 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPW86_RS02735 537022..537273 + 252 WP_123011643.1 hypothetical protein -
GPW86_RS02740 537270..537485 + 216 WP_123011644.1 hypothetical protein -
GPW86_RS02745 537634..538341 + 708 WP_001893912.1 HAD family hydrolase -
GPW86_RS02750 538467..538733 + 267 WP_000589156.1 BrnT family toxin -
GPW86_RS02755 538720..538965 + 246 WP_000643600.1 hypothetical protein -
GPW86_RS02760 539143..539427 + 285 WP_000048765.1 antibiotic biosynthesis monooxygenase -
GPW86_RS02765 539565..539834 - 270 WP_000277238.1 type II toxin-antitoxin system YafQ family toxin -
GPW86_RS02770 539827..540105 - 279 WP_001258569.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
GPW86_RS02775 540588..540638 + 51 Protein_531 DUF645 family protein -
GPW86_RS02785 540921..541208 + 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPW86_RS02790 541219..541536 + 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
GPW86_RS19365 541837..542035 + 199 Protein_534 DUF645 family protein -
GPW86_RS02805 542377..542556 + 180 WP_032470611.1 DUF645 family protein -
GPW86_RS02810 542798..543121 + 324 WP_080476600.1 DUF3709 domain-containing protein -
GPW86_RS02815 543440..543955 + 516 WP_000284464.1 hypothetical protein -
GPW86_RS02820 544012..544206 + 195 WP_123011647.1 DUF3709 domain-containing protein -
GPW86_RS02825 544242..544460 + 219 WP_184476335.1 DUF3709 domain-containing protein -
GPW86_RS02830 544729..545288 + 560 Protein_540 nucleotidyltransferase domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 444653..571022 126369
- inside Genomic island - - 443489..637173 193684


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-95)

Antitoxin

(12-69)


Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T144856 WP_001162670.1 NZ_CP046741:540921-541208 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T144856 NZ_CP062243:4439716-4440066 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT144856 WP_001232701.1 NZ_CP046741:541219-541536 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT144856 NZ_CP062243:4439471-4439722 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36


Antitoxin

Source ID Structure

References