Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 971254..971869 Replicon chromosome
Accession NZ_CP046738
Organism Vibrio cholerae strain 2015V-1126

Toxin (Protein)


Gene name higB Uniprot ID Q9KMG5
Locus tag GPW74_RS17370 Protein ID WP_001162670.1
Coordinates 971582..971869 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GPW74_RS17365 Protein ID WP_001232701.1
Coordinates 971254..971571 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPW74_RS18705 966790..967170 - 381 WP_055049814.1 hypothetical protein -
GPW74_RS17330 967401..967763 - 363 WP_162796981.1 DUF4144 domain-containing protein -
GPW74_RS17335 967803..968090 - 288 WP_080314578.1 DUF645 family protein -
GPW74_RS17340 968387..968773 - 387 WP_184478419.1 VOC family protein -
GPW74_RS17345 968922..969377 - 456 WP_001245329.1 GNAT family N-acetyltransferase -
GPW74_RS17350 969563..969943 - 381 WP_148038997.1 hypothetical protein -
GPW74_RS17355 969940..970500 - 561 WP_114794436.1 SocA family protein -
GPW74_RS17360 970779..970949 - 171 WP_000483442.1 DUF645 family protein -
GPW74_RS17365 971254..971571 - 318 WP_001232701.1 HigA family addiction module antidote protein Antitoxin
GPW74_RS17370 971582..971869 - 288 WP_001162670.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPW74_RS17375 972090..972869 - 780 WP_000333562.1 hypothetical protein -
GPW74_RS17385 973070..973315 + 246 WP_123012069.1 type II toxin-antitoxin system CcdA family antitoxin -
GPW74_RS17390 973315..973632 + 318 WP_000077449.1 CcdB family protein -
GPW74_RS17400 973749..974387 - 639 WP_032475759.1 HD domain-containing protein -
GPW74_RS17405 974535..975068 - 534 WP_123012070.1 GNAT family N-acetyltransferase -
GPW74_RS17410 975231..975647 - 417 WP_000759008.1 DUF1311 domain-containing protein -
GPW74_RS17415 975845..976015 - 171 Protein_826 DUF645 family protein -
GPW74_RS17420 976377..976538 - 162 WP_184478421.1 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 949375..1072270 122895
- inside Genomic island blaCARB-7 - 949582..1075528 125946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(2-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11349.95 Da        Isoelectric Point: 6.7187

>T144836 WP_001162670.1 NZ_CP046738:c971869-971582 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Download         Length: 288 bp

>T144836 NZ_CP062243:c1264731-1264624 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11705.63 Da        Isoelectric Point: 10.4711

>AT144836 WP_001232701.1 NZ_CP046738:c971571-971254 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Download         Length: 318 bp

>AT144836 NZ_CP062243:1264779-1264845 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F2HI36

Antitoxin

Source ID Structure

References