Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2258477..2259396 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag GP477_RS10520 Protein ID WP_003900449.1
Coordinates 2258791..2259396 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag GP477_RS10515 Protein ID WP_003410124.1
Coordinates 2258477..2258782 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS10480 2253704..2253979 + 276 WP_016330411.1 hypothetical protein -
GP477_RS10490 2255323..2255673 + 351 WP_003918359.1 hypothetical protein -
GP477_RS10495 2255670..2256710 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
GP477_RS10500 2256952..2257671 + 720 WP_003410108.1 DUF433 domain-containing protein -
GP477_RS10505 2257661..2258077 + 417 WP_003410114.1 hypothetical protein -
GP477_RS10510 2258093..2258392 - 300 WP_003410120.1 hypothetical protein -
GP477_RS10515 2258477..2258782 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
GP477_RS10520 2258791..2259396 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GP477_RS10525 2259421..2259780 - 360 WP_003410131.1 hypothetical protein -
GP477_RS10530 2259940..2260617 - 678 WP_003905725.1 hypothetical protein -
GP477_RS10535 2260569..2260805 - 237 WP_003410133.1 hypothetical protein -
GP477_RS10540 2261012..2261908 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T144798 WP_003900449.1 NZ_CP046728:c2259396-2258791 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T144798 NZ_CP062241:c3227814-3227500 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT144798 WP_003410124.1 NZ_CP046728:c2258782-2258477 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT144798 NZ_CP062241:c3227503-3227087 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References