Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2189353..2190221 Replicon chromosome
Accession NZ_CP046728
Organism Mycobacterium tuberculosis strain TCDC11

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag GP477_RS10120 Protein ID WP_010886136.1
Coordinates 2189353..2189730 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag GP477_RS10125 Protein ID WP_003409886.1
Coordinates 2189772..2190221 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GP477_RS10070 2185142..2185594 - 453 WP_003899095.1 lipoprotein -
GP477_RS10075 2185658..2186059 + 402 WP_003409869.1 hypothetical protein -
GP477_RS10080 2186052..2186234 - 183 WP_003409870.1 hypothetical protein -
GP477_RS10085 2186348..2186698 - 351 WP_003409871.1 hypothetical protein -
GP477_RS10090 2186709..2187611 - 903 WP_003899097.1 hypothetical protein -
GP477_RS10095 2187632..2187823 - 192 WP_003409876.1 hypothetical protein -
GP477_RS10100 2187824..2188120 - 297 WP_003409877.1 hypothetical protein -
GP477_RS10105 2188360..2188575 + 216 WP_003409878.1 antitoxin -
GP477_RS10110 2188572..2188883 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
GP477_RS10115 2188857..2189378 - 522 WP_003904745.1 hypothetical protein -
GP477_RS10120 2189353..2189730 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
GP477_RS10125 2189772..2190221 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
GP477_RS10130 2190218..2190763 + 546 WP_003409891.1 SecB-like chaperone -
GP477_RS10135 2190652..2191266 - 615 WP_003901296.1 hypothetical protein -
GP477_RS10140 2191315..2191611 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
GP477_RS10145 2191608..2191859 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
GP477_RS10150 2191846..2192340 + 495 WP_003899099.1 hypothetical protein -
GP477_RS10155 2192500..2192907 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
GP477_RS10160 2192911..2193183 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
GP477_RS10165 2193216..2194436 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T144793 WP_010886136.1 NZ_CP046728:2189353-2189730 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T144793 NZ_CP062241:2759277-2760350 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT144793 WP_003409886.1 NZ_CP046728:2189772-2190221 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT144793 NZ_CP062241:2760343-2760714 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References