Detailed information of TA system TA144757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 198997..199818
• Replicon: plasmid pASB05p1
• Accession: NZ_CP046723
• Organism: Pantoea agglomerans strain ASB05

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: PAASB05_RS19605
• Protein ID: WP_010670281.1
• Coordinates: 199348..199818 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: PAASB05_RS19600
• Protein ID: WP_158132276.1
• Coordinates: 198997..199341 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAASB05_RS19580	194998..196107	+	1110	WP_031594250.1	maltose/maltodextrin ABC transporter ATP-binding protein MalK	-
PAASB05_RS19585	196151..197452	+	1302	WP_158132273.1	maltoporin	-
PAASB05_RS19590	197555..198493	+	939	WP_158132274.1	maltose operon protein MalM	-
PAASB05_RS19595	198682..198897	+	216	WP_158132275.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
PAASB05_RS19600	198997..199341	+	345	WP_158132276.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
PAASB05_RS19605	199348..199818	+	471	WP_010670281.1	type II toxin-antitoxin system YhaV family toxin	Toxin
PAASB05_RS19610	200006..202081	-	2076	WP_158132277.1	4-alpha-glucanotransferase	-
PAASB05_RS19615	202090..204492	-	2403	WP_158132278.1	maltodextrin phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..563807	563807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (13-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 18238.78 Da
• Isoelectric Point: 8.9262

>T144757 WP_010670281.1 NZ_CP046723:199348-199818 [Pantoea agglomerans]
MEDSTVINGWHVYVPPCFESQLIALTNEVELLRAKYPESYQRKATTKLLAAVFKVINSEICADPHQAKFRQGDTLGEQNK
HWFRAKFLMQYRLFFRFSEQHKTIILAWMNDAETKRAYGSKRDAYKVFSGMLHNGYPPDDWALLLKQSQDWANTNS

Nucleotide

• Length: 471 bp

>T144757 NZ_CP062240:c1265801-1265694 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12242.98 Da
• Isoelectric Point: 4.5917

>AT144757 WP_158132276.1 NZ_CP046723:198997-199341 [Pantoea agglomerans]
MGTLTQANISLRASSKLTARGQTTIPAAVRDAMNLQPGENIEYAVLPGGKVLITRQETVADDAPVKASFLSFLPADRRNT
AANIKMLDRGLLTRITSLTEGMDVDLDAPLMDDE

Nucleotide

• Length: 345 bp

>AT144757 NZ_CP062240:1265848-1265914 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT