Detailed information of TA system TA144749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 1534762..1536092
• Replicon: chromosome
• Accession: NZ_CP046722
• Organism: Pantoea agglomerans strain ASB05

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: PAASB05_RS07050
• Protein ID: WP_158131579.1
• Coordinates: 1534762..1535784 (-)
• Length: 341 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A7X2SVD5
• Locus tag: PAASB05_RS07055
• Protein ID: WP_010670935.1
• Coordinates: 1535781..1536092 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAASB05_RS07045	1532459..1534636	+	2178	WP_158132158.1	catalase/peroxidase HPI	-
PAASB05_RS07050	1534762..1535784	-	1023	WP_158131579.1	HipA domain-containing protein	Toxin
PAASB05_RS07055	1535781..1536092	-	312	WP_010670935.1	HipA N-terminal domain-containing protein	Antitoxin
PAASB05_RS07060	1536077..1536421	-	345	WP_010247978.1	helix-turn-helix transcriptional regulator	-
PAASB05_RS07070	1537199..1538320	+	1122	WP_158131580.1	Gfo/Idh/MocA family oxidoreductase	-
PAASB05_RS07075	1538411..1539241	-	831	WP_060680551.1	glycosyltransferase family 8 protein	-
PAASB05_RS07080	1539254..1540636	-	1383	WP_132500078.1	FAD-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (58-302)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 341 a.a.
• Molecular weight: 38563.14 Da
• Isoelectric Point: 6.8346

>T144749 WP_158131579.1 NZ_CP046722:c1535784-1534762 [Pantoea agglomerans]
MNSERCLITLKRLKESELKTGYSTKGIRHLLGSLNVALSLPFTRSQFIQELPERQKGMSISGYQPKLSLRLTKGELVVVS
NDGTYILKPSPEEYPHLAENEHATMCVMQRLKFDVPPFGLMPFRHDENRPEELAFVIKRYDRTASDNARLHQEQLDGAMG
LGDKYGRIDNHKAISYEKAAKFLNTRVNSSLKSKRDLFLRIVYAYVLGNNDFHLRNIGIILPPQGSPWLAPVYDFISVVP
YPAAFGEYLALPLLEREENDAGNAPGINSEYGEYLGADFILLAQGMDINPTLARKWLSEVVSHHQLIIDTYNESHMPESQ
REAVLDYVARRITLLAVTEI

Nucleotide

• Length: 1023 bp

>T144749 NZ_CP062239:c4523870-4523613 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11668.44 Da
• Isoelectric Point: 4.7953

>AT144749 WP_010670935.1 NZ_CP046722:c1536092-1535781 [Pantoea agglomerans]
MARKIEVWLYNTLAGTLSEGQNGYTFCYLPTYAGCAISLSMPVRPEPYLSEELHPFFKGLAPEGWLRKRYSEIQKIDDKD
LFGLLMENGNDLLGAVVFKEITS

Nucleotide

• Length: 312 bp

>AT144749 NZ_CP062239:c4523601-4523056 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X2SVD5