Detailed information of TA system TA144742

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 143097..143523
• Replicon: plasmid pT16R-2
• Accession: NZ_CP046718
• Organism: Escherichia coli strain T16R

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GPL39_RS24110
• Protein ID: WP_001312861.1
• Coordinates: 143097..143255 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	143299..143523 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPL39_RS24090	138602..139423	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GPL39_RS24095	139545..139832	-	288	WP_000107535.1	hypothetical protein	-
GPL39_RS24615	139857..140063	-	207	WP_000547939.1	hypothetical protein	-
GPL39_RS24105	141035..142702	+	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
GPL39_RS24110	143097..143255	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	143299..143523	-	225	NuclAT_0	-	Antitoxin
-	143299..143523	-	225	NuclAT_0	-	Antitoxin
-	143299..143523	-	225	NuclAT_0	-	Antitoxin
-	143299..143523	-	225	NuclAT_0	-	Antitoxin
GPL39_RS24115	143335..143523	+	189	WP_001299721.1	hypothetical protein	-
GPL39_RS24120	143535..144254	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
GPL39_RS24125	144251..144685	-	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
GPL39_RS24130	144740..146698	-	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
GPL39_RS24135	146764..146997	-	234	WP_000005990.1	DUF905 family protein	-
GPL39_RS24140	147060..147599	-	540	WP_000290791.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / aph(3')-Ia / blaTEM-1A / floR / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN	1..172892	172892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T144742 WP_001312861.1 NZ_CP046718:c143255-143097 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T144742 NZ_CP062239:c3034182-3033775 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT144742 NZ_CP046718:c143523-143299 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin