Detailed information of TA system TA144739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3661840..3662534
• Replicon: chromosome
• Accession: NZ_CP046716
• Organism: Escherichia coli strain T16R

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GPL39_RS17720
• Protein ID: WP_001263493.1
• Coordinates: 3661840..3662238 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GPL39_RS17725
• Protein ID: WP_000554757.1
• Coordinates: 3662241..3662534 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPL39_RS17690	3656852..3658096	-	1245	WP_000189541.1	esterase FrsA	-
-	3657512..3657592	-	81	NuclAT_11	-	-
-	3657512..3657592	-	81	NuclAT_11	-	-
-	3657512..3657592	-	81	NuclAT_11	-	-
-	3657512..3657592	-	81	NuclAT_11	-	-
GPL39_RS17695	3658188..3658646	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GPL39_RS17700	3658907..3660364	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
GPL39_RS17705	3660421..3660930	-	510	Protein_3456	peptide chain release factor H	-
GPL39_RS17710	3660929..3661132	-	204	Protein_3457	RNA ligase RtcB family protein	-
GPL39_RS17715	3661378..3661830	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GPL39_RS17720	3661840..3662238	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GPL39_RS17725	3662241..3662534	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GPL39_RS17730	3662586..3663641	-	1056	WP_063501914.1	DNA polymerase IV	-
GPL39_RS17735	3663712..3664497	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
GPL39_RS17740	3664469..3666181	+	1713	Protein_3463	flagellar biosynthesis protein FlhA	-
GPL39_RS17745	3666405..3666902	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T144739 WP_001263493.1 NZ_CP046716:c3662238-3661840 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T144739 NZ_CP062239:2759277-2760350 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT144739 WP_000554757.1 NZ_CP046716:c3662534-3662241 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT144739 NZ_CP062239:2760343-2760714 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6