Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1749700..1750202 Replicon chromosome
Accession NZ_CP046716
Organism Escherichia coli strain T16R

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GPL39_RS08230 Protein ID WP_000767829.1
Coordinates 1749948..1750202 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GPL39_RS08225 Protein ID WP_001259255.1
Coordinates 1749700..1749951 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPL39_RS08200 1744878..1745945 - 1068 WP_148245887.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
GPL39_RS08205 1745945..1747015 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
GPL39_RS08210 1747012..1748316 - 1305 WP_137456045.1 histidinol dehydrogenase -
GPL39_RS08215 1748322..1749221 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
GPL39_RS08220 1749367..1749417 - 51 WP_001364200.1 his operon leader peptide -
GPL39_RS08225 1749700..1749951 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GPL39_RS08230 1749948..1750202 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GPL39_RS08235 1750285..1751109 + 825 WP_000754737.1 SDR family oxidoreductase -
GPL39_RS08240 1751155..1752084 + 930 WP_000803351.1 LysR family transcriptional regulator -
GPL39_RS08245 1752299..1752361 + 63 WP_010723108.1 membrane protein YoeI -
GPL39_RS08250 1752351..1753709 + 1359 WP_024198437.1 putrescine/proton symporter PlaP -
GPL39_RS08255 1753888..1754946 + 1059 WP_074511709.1 transport protein YeeE -
GPL39_RS08260 1754960..1755187 + 228 WP_096310218.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T144727 WP_000767829.1 NZ_CP046716:1749948-1750202 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T144727 NZ_CP062239:c475764-475546 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT144727 WP_001259255.1 NZ_CP046716:1749700-1749951 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT144727 NZ_CP062239:c476164-475790 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References