Detailed information of TA system TA144721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 661442..662169
• Replicon: chromosome
• Accession: NZ_CP046716
• Organism: Escherichia coli strain T16R

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5P0J3S6
• Locus tag: GPL39_RS03200
• Protein ID: WP_063501682.1
• Coordinates: 661442..661756 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GPL39_RS03205
• Protein ID: WP_000560266.1
• Coordinates: 661753..662169 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPL39_RS03180	657600..658586	-	987	WP_000617698.1	Gfo/Idh/MocA family oxidoreductase	-
GPL39_RS03185	658665..659357	-	693	WP_063501683.1	vancomycin high temperature exclusion protein	-
GPL39_RS03190	659434..659937	-	504	WP_001333820.1	M48 family metallopeptidase	-
GPL39_RS03195	660022..661158	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
GPL39_RS03200	661442..661756	+	315	WP_063501682.1	type II toxin-antitoxin system toxin HigB	Toxin
GPL39_RS03205	661753..662169	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
GPL39_RS03210	662214..664232	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
GPL39_RS03215	664658..667009	-	2352	WP_000695487.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12202.24 Da
• Isoelectric Point: 10.1604

>T144721 WP_063501682.1 NZ_CP046716:661442-661756 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALRNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T144721 NZ_CP062231:c4230363-4229857 [Rhizobium sp. TH2]
ATGGCTGACCAAGCGCGGTTCGACCATCCGGTTGTTATCGATCCTGATCACGATCTGTTGTCATTTCAATGCGGCGAACC
CATCCTCGATGATTGGCTCAAAACACGCGCGCTCAAGAACCTTGCGCTGGGTGCAAGCAGGACATTTGTGACCTGTCACG
CGGGTTCGAACAGAGTGGTGGGCTATTACGCACTTTCGATGGGTAGCCTGTTTGCGATCGACACGCCTGGTAGCATTCGC
AGGAATATGCCGGAGATCATTCCATCGGTTGTTCTCGGTCGACTTGCAATCGACGAAACCGCCCAGGGTCACGGCCTCGG
CGCGGCCTTGTTGCACGACGCTGTCAATCGTAGCCTCGTGGCTTCCCAGAATGTCAGTGCCCGCCTAGTCGTGGTGCATG
CCATCTCGCAGAGAGCGGAACAGTTCTACCTCCGCTTTGGATTCGTCAGAATGCCGGTGGAAGACAGGACCCTGGCGCTC
GATTTGGTCAAATACCAAAAACGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT144721 WP_000560266.1 NZ_CP046716:661753-662169 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT144721 NZ_CP062231:c4230646-4230356 [Rhizobium sp. TH2]
ATGGCTGGACAACCCGAAACATCCGAAGTCAATATTCATCTCCGCGCCCGGTCGGGCGATCGGGATTTGATCGATCGCGC
CGCAAATATTTCGGGGACCAACCGGTCGCAGTTCATGCTGTCATCTGCACTCAAGGAAGCCACAAACGTCCTTCTTGACC
AGACGATGATTCGTCTTGATGCCGAAGCGTTCCAAAAAGTGCTGACTTGGATGGACGCGGTTCCTTCGGAGGCTGAATCC
AAGGGCATCGACCGCCTTAAATCTGTCAAATCAGATTGGGCCAATGGCTGA

Structures

Toxin

• AlphaFold DB: A0A5P0J3S6