Detailed information of TA system TA144715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1542039..1542691
• Replicon: plasmid pRS
• Accession: NZ_CP046675
• Organism: Ralstonia nicotianae strain RS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: GO999_RS22755
• Protein ID: WP_028861315.1
• Coordinates: 1542039..1542383 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GO999_RS22760
• Protein ID: WP_020425351.1
• Coordinates: 1542380..1542691 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO999_RS22740 (GO999_22715)	1537734..1538897	-	1164	WP_211906911.1	2-methylcitrate synthase	-
GO999_RS22745 (GO999_22720)	1538949..1539845	-	897	WP_020425352.1	methylisocitrate lyase	-
GO999_RS22750 (GO999_22725)	1540039..1541988	+	1950	WP_211906912.1	propionate catabolism operon regulatory protein PrpR	-
GO999_RS22755 (GO999_22730)	1542039..1542383	+	345	WP_028861315.1	hypothetical protein	Toxin
GO999_RS22760 (GO999_22735)	1542380..1542691	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
GO999_RS22765 (GO999_22740)	1542831..1543640	-	810	WP_043885575.1	DUF2242 domain-containing protein	-
GO999_RS22770 (GO999_22745)	1544927..1546723	+	1797	WP_249215089.1	3'-5' exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / adeG / cheY / cheB / cheW / motA / cyaB / flgC / flgG / fliM / fliG / fliI	1..1994515	1994515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T144715 WP_028861315.1 NZ_CP046675:1542039-1542383 [Ralstonia nicotianae]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T144715 NZ_CP062231:c1111359-1110985 [Rhizobium sp. TH2]
ATGACGAAGCCTCCTTTTCAATATGGTGAAAAGCCCCTCCATTGGGTGGGCTCGTCGAAGCGGGATTTCATGGCGTTCCC
GCAAGAGGTCATCAGCGGCATGGGCTATGCGCTCGGCCTTGCACAACTCGGCGGAAAACATCCGCATGCCAAGCCATGGA
AAGGCGAAGGCCCGGGTGTCCTCGAAATCGTCGAGAACCATGATGGCGATACATATCGCGCCGTCTATACCGTTCGGTTC
GAAGCAGCGATCTATGTGTTGCATGCATTCCAGAAGAAGAGTCCTTCGGGAATACGCACCGCGACGAGCGACATCGATCT
CATTGGTGAGAGATTGAAACGGGCCAGGGAAGATCACGAAAGATCGAGAAAGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT144715 WP_020425351.1 NZ_CP046675:1542380-1542691 [Ralstonia nicotianae]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT144715 NZ_CP062231:c1110973-1110656 [Rhizobium sp. TH2]
ATGGACATCGAATACGGCAGCACGAATGTTTTCGCCGATCTCGGCCTTCCGGATGCTGTCGGGCGGCAGACCAAGACGCG
TCTTGCGCTTGAGATCAATACGATCCTCAAGGGGCGCAAGCTTCGACAGACGTCAGCGGCATCCCTGCTCGGAATCCCGC
AGTCCAAAGTGTCCGCTCTGGTCAACTATCGGCTGGAAGGGTTTTCTGTCGAAAGGCTCATGGGCTTTCTCACGTCGCTC
AATCAGGATATCGAGATCATGATCAGGCCGAGCCGCAGTGAAAAGGAAGGGCATGTTTCGGTGCATGCGCTTCGGTAA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4