Detailed information of TA system TA144662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2901..3552
• Replicon: plasmid pMAC
• Accession: NZ_CP046655
• Organism: Acinetobacter baumannii strain ATCC 19606

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: GO593_RS18980
• Protein ID: WP_000269904.1
• Coordinates: 2901..3257 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GO593_RS18985
• Protein ID: WP_001129974.1
• Coordinates: 3250..3552 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO593_RS18960	44..979	+	936	WP_001292329.1	replication initiation protein RepM	-
GO593_RS18965	1057..1575	+	519	WP_000839337.1	plasmid replication DNA-binding protein	-
GO593_RS18980	2901..3257	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GO593_RS18985	3250..3552	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
GO593_RS18990	3545..3754	+	210	WP_000069474.1	hypothetical protein	-
GO593_RS18995	3929..4447	+	519	WP_000447196.1	sel1 repeat family protein	-
GO593_RS19000	4486..4998	+	513	WP_001089573.1	SMI1/KNR4 family protein	-
GO593_RS19005	5196..5624	+	429	WP_000105988.1	organic hydroperoxide resistance protein	-
GO593_RS19010	5631..6062	+	432	WP_000042163.1	MarR family transcriptional regulator	-
GO593_RS19015	6295..6522	-	228	WP_000555568.1	hypothetical protein	-
GO593_RS19020	6752..7513	-	762	WP_000425105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9540	9540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T144662 WP_000269904.1 NZ_CP046655:2901-3257 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T144662 NZ_CP062211:c3833892-3833485 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT144662 WP_001129974.1 NZ_CP046655:3250-3552 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT144662 NZ_CP062211:c3834139-3833873 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: N8YFZ1