Detailed information of TA system TA144648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3929365..3929962
• Replicon: chromosome
• Accession: NZ_CP046645
• Organism: Klebsiella pneumoniae strain EGKP3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: GPU01_RS19080
• Protein ID: WP_004893639.1
• Coordinates: 3929645..3929962 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: GPU01_RS19075
• Protein ID: WP_004142561.1
• Coordinates: 3929365..3929652 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPU01_RS19045 (GPU01_18765)	3925445..3925693	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
GPU01_RS19050 (GPU01_18770)	3925711..3926052	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
GPU01_RS19055 (GPU01_18775)	3926083..3927198	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
GPU01_RS19060 (GPU01_18780)	3927378..3927959	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
GPU01_RS19065 (GPU01_18785)	3927959..3928327	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
GPU01_RS19070 (GPU01_18790)	3928447..3929100	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
GPU01_RS19075 (GPU01_18795)	3929365..3929652	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
GPU01_RS19080 (GPU01_18800)	3929645..3929962	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GPU01_RS19085 (GPU01_18805)	3930147..3931190	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
GPU01_RS19090 (GPU01_18810)	3931856..3932722	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
GPU01_RS19095 (GPU01_18815)	3932831..3934258	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T144648 WP_004893639.1 NZ_CP046645:c3929962-3929645 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T144648 NZ_CP062211:c959508-958966 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT144648 WP_004142561.1 NZ_CP046645:c3929652-3929365 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT144648 NZ_CP062211:c959803-959492 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1