Detailed information of TA system TA144604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 117660..118387
• Replicon: plasmid pWCGKP294-1
• Accession: NZ_CP046613
• Organism: Klebsiella pneumoniae strain WCGKP294

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: GPJ73_RS27470
• Protein ID: WP_011251285.1
• Coordinates: 118076..118387 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GPJ73_RS27465
• Protein ID: WP_011251286.1
• Coordinates: 117660..118079 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPJ73_RS27440	113252..114619	+	1368	WP_017900870.1	formimidoylglutamate deiminase	-
GPJ73_RS27445	115144..115679	+	536	Protein_111	transposase	-
GPJ73_RS27450	115793..116059	-	267	WP_017900868.1	hypothetical protein	-
GPJ73_RS27455	116163..117131	+	969	WP_074168518.1	IS5-like element IS903B family transposase	-
GPJ73_RS27460	117177..117590	-	414	WP_017901337.1	hypothetical protein	-
GPJ73_RS27465	117660..118079	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
GPJ73_RS27470	118076..118387	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
GPJ73_RS27475	118592..119050	-	459	Protein_117	DDE-type integrase/transposase/recombinase	-
GPJ73_RS27480	119143..119520	+	378	WP_004118218.1	IS66 family insertion sequence hypothetical protein	-
GPJ73_RS27485	119517..119864	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
GPJ73_RS27490	119913..121451	+	1539	WP_017901237.1	IS66 family transposase	-
GPJ73_RS27495	121491..121670	+	180	Protein_121	IS5/IS1182 family transposase	-
GPJ73_RS27500	122216..123196	-	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-12	-	1..237090	237090
	-	inside	IScluster/Tn	-	-	115285..123196	7911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T144604 WP_011251285.1 NZ_CP046613:c118387-118076 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T144604 NZ_CP062203:c827531-827154 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT144604 WP_011251286.1 NZ_CP046613:c118079-117660 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT144604 NZ_CP062203:c827989-827621 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1