Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 364347..364993 Replicon chromosome
Accession NZ_CP046612
Organism Klebsiella pneumoniae strain WCGKP294

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag GPJ73_RS01670 Protein ID WP_016529833.1
Coordinates 364347..364694 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag GPJ73_RS01675 Protein ID WP_002920557.1
Coordinates 364694..364993 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ73_RS01660 360283..361716 + 1434 WP_002920564.1 glycogen synthase GlgA -
GPJ73_RS01665 361734..364181 + 2448 WP_002920561.1 glycogen phosphorylase -
GPJ73_RS01670 364347..364694 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPJ73_RS01675 364694..364993 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
GPJ73_RS01680 365056..366564 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
GPJ73_RS01685 366769..367098 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
GPJ73_RS01690 367149..367979 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
GPJ73_RS01695 368029..368787 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T144585 WP_016529833.1 NZ_CP046612:364347-364694 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T144585 NZ_CP062160:c5409823-5409548 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT144585 WP_002920557.1 NZ_CP046612:364694-364993 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT144585 NZ_CP062160:c5409551-5409192 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References