Detailed information of TA system TA144571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4369683..4370305
• Replicon: chromosome
• Accession: NZ_CP046602
• Organism: Pseudomonas aeruginosa strain CMC-115

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GO602_RS20360
• Protein ID: WP_023910260.1
• Coordinates: 4369991..4370305 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GO602_RS20355
• Protein ID: WP_023910258.1
• Coordinates: 4369683..4369988 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO602_RS20310	4365260..4365640	+	381	WP_033987591.1	response regulator transcription factor	-
GO602_RS20315	4365643..4365876	+	234	WP_033987589.1	hypothetical protein	-
GO602_RS20320	4365974..4366204	+	231	WP_033987588.1	Arc family DNA-binding protein	-
GO602_RS20325	4366207..4366542	+	336	WP_033987586.1	hypothetical protein	-
GO602_RS20330	4366539..4367810	+	1272	WP_168334434.1	hypothetical protein	-
GO602_RS20335	4367923..4368114	+	192	WP_033987585.1	hypothetical protein	-
GO602_RS20340	4368287..4368976	+	690	WP_033987583.1	hypothetical protein	-
GO602_RS20345	4368981..4369322	+	342	WP_033987626.1	hypothetical protein	-
GO602_RS20350	4369319..4369621	+	303	WP_033987582.1	hypothetical protein	-
GO602_RS20355	4369683..4369988	-	306	WP_023910258.1	helix-turn-helix domain-containing protein	Antitoxin
GO602_RS20360	4369991..4370305	-	315	WP_023910260.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GO602_RS20365	4370302..4370649	-	348	WP_079381619.1	hypothetical protein	-
GO602_RS20370	4371061..4372224	-	1164	WP_071538382.1	hypothetical protein	-
GO602_RS20375	4372444..4373094	-	651	WP_033987580.1	BRCT domain-containing protein	-
GO602_RS20380	4373213..4373524	-	312	WP_031631670.1	outer membrane protein assembly factor BamE	-
GO602_RS20385	4373593..4374591	-	999	WP_033987579.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4332807..4411258	78451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11776.54 Da
• Isoelectric Point: 9.4385

>T144571 WP_023910260.1 NZ_CP046602:c4370305-4369991 [Pseudomonas aeruginosa]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGVVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLAY
DKASQEDLTADQKKMLRQIVENWR

Nucleotide

• Length: 315 bp

>T144571 NZ_CP062160:2436907-2437010 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11316.84 Da
• Isoelectric Point: 7.2077

>AT144571 WP_023910258.1 NZ_CP046602:c4369988-4369683 [Pseudomonas aeruginosa]
MDKELFAELVESVTQMDEIVRGERQPSREFHVDAVKVKEIRRATGLSQAKFARKIDVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRES

Nucleotide

• Length: 306 bp

>AT144571 NZ_CP062160:c2437048-2436777 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT