Detailed information of TA system TA144529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1442691..1443385
• Replicon: chromosome
• Accession: NZ_CP046582
• Organism: Erwinia sp. E602

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: GKQ23_RS08050
• Protein ID: WP_212411676.1
• Coordinates: 1442987..1443385 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: GKQ23_RS08045
• Protein ID: WP_056239744.1
• Coordinates: 1442691..1442984 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ23_RS08025 (GKQ23_07995)	1438210..1438491	+	282	WP_056239369.1	YfcL family protein	-
GKQ23_RS08030 (GKQ23_08000)	1438677..1440686	-	2010	WP_212410232.1	bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC	-
GKQ23_RS08035 (GKQ23_08005)	1440842..1442062	+	1221	WP_056239376.1	beta-ketoacyl-ACP synthase I	-
GKQ23_RS08040	1442294..1442551	-	258	WP_212410233.1	YceK/YidQ family lipoprotein	-
GKQ23_RS08045 (GKQ23_08015)	1442691..1442984	+	294	WP_056239744.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GKQ23_RS08050 (GKQ23_08020)	1442987..1443385	+	399	WP_212411676.1	type II toxin-antitoxin system YafO family toxin	Toxin
GKQ23_RS08055 (GKQ23_08025)	1443696..1444874	+	1179	WP_212410234.1	MFS transporter	-
GKQ23_RS08060 (GKQ23_08030)	1444862..1445023	-	162	WP_212410235.1	hypothetical protein	-
GKQ23_RS08065 (GKQ23_08035)	1445115..1445663	+	549	WP_212410236.1	rhodanese-like domain-containing protein	-
GKQ23_RS24050	1445660..1445794	-	135	WP_255354464.1	hypothetical protein	-
GKQ23_RS08070 (GKQ23_08040)	1445866..1446897	-	1032	WP_212410237.1	flagella biosynthesis regulator Flk	-
GKQ23_RS08075 (GKQ23_08045)	1447200..1448213	-	1014	WP_212410238.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (28-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15041.26 Da
• Isoelectric Point: 6.9784

>T144529 WP_212411676.1 NZ_CP046582:1442987-1443385 [Erwinia sp. E602]
IRVFKSTLISQQLAPQELEDLVADFRGYKQDGVLPDTFGRDAPYDDAATYPLVKREEVAHLHLADASAPFPKFLRQFRRT
SDSAHLVYCQGAMESDAYLLIIILKPDAHKMARDNNNMHKIGLMAEAFRMKF

Nucleotide

• Length: 399 bp

>T144529 NZ_CP062122:c4817006-4816593 [Pseudomonas sp. ADPe]
ATGCTGAAGTTCATGCTCGATACCAACATCTGCATCTTCACCATCAAGAACAAGCCGCAAGTCGTTCGCGAGGCCTTCAA
CCAGCACCATGGGCAGTTGGCGATCAGTTCGGTGACGCTGATGGAACTGATATACGGCGCCGAGAAGTCCTCCATGCCGG
AACACAACCTGGGCATCGTCGAAGGCTTCGCCGCACGCCTGGAAGTGCTCGACTACGACGGCAACGCGGCGGCCCACACC
GGGCAACTGCGCGCCGAGCTGGCGCGAATCGGCAAGCCCATCGACCCCTACGACCAGATGATCGCCGGGCACGCCCGTTC
CCGCGGGCTGATCCTGGTCAGCAACAACCTGCGCGAATTCGAGCGAGTGCCGGGGCTGCGCCTGGAGGACTGGACCGTAG
CCCCATCGCCTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11106.56 Da
• Isoelectric Point: 4.7147

>AT144529 WP_056239744.1 NZ_CP046582:1442691-1442984 [Erwinia sp. E602]
MERILAEKTINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSARVFEELIDMLQDKQETVHGLARFRPNAARLNEITASG
QELLDRASDNDLREFTE

Nucleotide

• Length: 294 bp

>AT144529 NZ_CP062122:c4817236-4817006 [Pseudomonas sp. ADPe]
ATGGAACAAGGCGCCGTATTCCAGAGCAACCGCAGCCAGGCCGTGCGCCTGCCCAAGGCCGTCGCGCTGCCTGAAGACGT
GAAGCGCGTAGACGTCGTGGCCATCGGCCGAACCCGCATCCTCACCCCGGCCGGGGAGAGCTGGGACAGCTGGTTCGACG
GCGAGAACGTCAGCGCCGACTTCATGAGCGAGCGCGAGCAACCGGCGGAACAGGAACGCGAGGGGTTCTGA