Detailed information of TA system TA144495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 60252..60898
• Replicon: chromosome
• Accession: NZ_CP046557
• Organism: Leclercia sp. 1106151

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GNG29_RS00310
• Protein ID: WP_103177685.1
• Coordinates: 60548..60898 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GNG29_RS00305
• Protein ID: WP_156653669.1
• Coordinates: 60252..60551 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG29_RS00280	55985..57073	-	1089	WP_156653667.1	AAA family ATPase	-
GNG29_RS00285	57218..58177	-	960	WP_103177681.1	DUF523 and DUF1722 domain-containing protein	-
GNG29_RS00290	58280..58690	-	411	WP_114385495.1	hydroxyisourate hydrolase	-
GNG29_RS00295	58787..59515	-	729	WP_156653668.1	MerR family transcriptional regulator	-
GNG29_RS00300	59633..60157	+	525	WP_103177683.1	lipocalin family protein	-
GNG29_RS00305	60252..60551	-	300	WP_156653669.1	XRE family transcriptional regulator	Antitoxin
GNG29_RS00310	60548..60898	-	351	WP_103177685.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GNG29_RS00315	61009..61446	+	438	WP_156653670.1	acetyltransferase	-
GNG29_RS00320	61519..62040	-	522	WP_103181009.1	hypothetical protein	-
GNG29_RS00325	62369..62656	-	288	WP_156653671.1	SymE family type I addiction module toxin	-
GNG29_RS00330	62785..62961	+	177	WP_156653672.1	DUF3950 domain-containing protein	-
GNG29_RS00335	63006..63698	-	693	WP_156653673.1	nucleotidyltransferase domain-containing protein	-
GNG29_RS00340	63695..64897	-	1203	WP_156653674.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13547.46 Da
• Isoelectric Point: 7.2761

>T144495 WP_103177685.1 NZ_CP046557:c60898-60548 [Leclercia sp. 1106151]
MWTVILGEVFEAWLEDQEQGVQEKVFAALINLQTYGPRLPRPYADTVRGSRHNNMKELRIQYYGRPIRAFFAFDPVRQAI
VLCAGDKSHDKKFYERLIRIADDEFSAHLAAMETRN

Nucleotide

• Length: 351 bp

>T144495 NZ_CP062005:1819866-1820189 [Halomonas sp. YLGW01]
ATGAGGCGTGGGGATCTCGTGACCATTGCCCTATCGGGTGATTTCGGTAAGCCGCGTCCCGCCCTCATCGTCCAGTCCGA
CCAGTTTGCCGGCACAGGCAGTGTGACGGTGCTGCTGCTCTCCAGAAGCCGGGTCAATGCACCGTTGATCCGCCTCGATG
TTGAGCCCACCCCCGAGAACGGCTTGCACCGACGCTCCCAGATCATGGTGGACAAACCCATGACGGTGAAACGCGAGAGA
GTTGGAGAGTACTTCGGTCACCTCGATGACAGCGCCATGGTCGCCGTCAACAGAGCGCTGGCCGTCTTCCTGGGCTTCGC
GTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11195.12 Da
• Isoelectric Point: 8.6049

>AT144495 WP_156653669.1 NZ_CP046557:c60551-60252 [Leclercia sp. 1106151]
MKTLNEAIASRSPESQKRIKEMADEMILETGLQMMREELKLSQKHVAEVMGISQPAITQLEQRGNELKITTLKRYIEAMG
GKLSLDVELPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT144495 NZ_CP062005:1819633-1819869 [Halomonas sp. YLGW01]
ATGGTGGCTACGAACGATGTAAACAACAGGGTGAAGAAGCACCGCGGCGCCTTGCGAGCGGCCGGCCTGCGCCCGATCCA
AATCTGGGTCCCGGACACTCGACAACCCGGCTTCGCCGAAGAGGCTCACCGTCAGTGCGCTCTCGTGGCAGCTGCCGACG
CCGGGGATCGCGACCTGCAGGACTTCATGGACGCGGCTCTGCTGGATCTTGACCTGGATGACGAGGGTGACGCATGA